Department of Pharmaceutical Chemistry, Friedrich Schiller University Jena, Jena, Germany.
Electrophoresis. 2011 Oct;32(19):2669-72. doi: 10.1002/elps.201000610. Epub 2011 Sep 8.
A stereoselective CE assay for the determination of the enantiomeric purity of (R)-(-)-chloroquine and (S)-(+)-chloroquine was developed and validated. The separations were performed in a 50.2/40 cm uncoated fused silica capillary at 20°C using a 100 mM sodium phosphate buffer, pH 2.5, containing 30 mg/mL sulfobutylether(VII)-β-cyclodextrin as background electrolyte operated at an applied voltage of -25 kV and 20°C. The detection wavelength was 225 nm. Carbamazepine was used as internal standard. The assay was validated in the range of 0.05-1.0% for the respective minor chloroquine enantiomer based on a concentration of 3 mg/mL of the major enantiomer, either (R)-(-)-chloroquine or (S)-(+)-chloroquine. The method was applied to analyze the stereoisomeric purity of synthetic samples of the chloroquine enantiomers.
建立并验证了一种用于测定（R）-（-）-氯喹和（S）-（+）-氯喹对映体纯度的手性毛细管电泳分析方法。在 20°C 下，使用未涂层的 50.2/40 cm 熔融石英毛细管，以 100 mM 磷酸钠缓冲液（pH 2.5）为背景电解质，其中含有 30 mg/mL 的磺丁基醚（VII）-β-环糊精，施加-25 kV 的电压和 20°C 的温度进行分离。检测波长为 225nm。卡马西平用作内标。该方法基于浓度为 3 mg/mL 的主要对映异构体（R）-（-）-氯喹或（S）-（+）-氯喹，在各自的少量氯喹对映异构体的 0.05-1.0%范围内进行验证。该方法应用于分析合成的氯喹对映异构体样品的立体异构体纯度。
