Department of Anatomy, College of Veterinary Medicine and Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, South Korea.
Am J Chin Med. 2011;39(5):971-9. doi: 10.1142/S0192415X11009342.
EGb 761 is an extract of Gingko biloba that is neuroprotective against focal cerebral ischemic injury. PEA-15 (phosphoprotein enriched in astrocytes 15) modulates cell proliferation and apoptosis. In this study, we investigated whether EGb 761 regulates the expression of PEA-15 and two phosphorylated forms of PEA-15 (Ser 104 and Ser 116) in middle cerebral artery occlusion (MCAO)-induced injury. Adult male rats were treated with vehicle or EGb 761 (100 mg/kg) prior to MCAO and cerebral cortices were collected 24 h after MCAO. A reduction in expression of PEA-15 and its phosphorylated forms induced by MCAO injury was detected using a proteomic approach. EGb 761 pretreatment prevented the ischemic injury-induced decrease in PEA-15 expression. Western blot analysis demonstrated that EGb 761 attenuates the injury-induced reduction in PEA-15, phospho-PEA-15 (Ser 104), phospho-PEA-15 (Ser 116). Phosphorylation of PEA-15 influences its anti-apoptotic function; a decrease in PEA-15 phosphorylation induces apoptotic cell death. The maintenance of PEA-15 phosphorylation by EGb 761 pretreatment during cerebral ischemic injury indicates that EGb 761 is a neuroprotective against cerebral ischemic injury.
银杏叶提取物 EGb 761 对大脑局部缺血性损伤具有神经保护作用。PEA-15（富含星形胶质细胞的磷蛋白 15）可调节细胞增殖和凋亡。在这项研究中，我们研究了 EGb 761 是否调节大脑中动脉闭塞（MCAO）诱导损伤中 PEA-15 及其两种磷酸化形式（Ser 104 和 Ser 116）的表达。成年雄性大鼠在 MCAO 前用载体或 EGb 761（100mg/kg）处理，并在 MCAO 后 24 小时收集大脑皮质。通过蛋白质组学方法检测 MCAO 损伤诱导的 PEA-15 和其磷酸化形式表达减少。EGb 761 预处理可预防缺血性损伤诱导的 PEA-15 表达减少。Western blot 分析表明，EGb 761 可减轻损伤诱导的 PEA-15、磷酸化 PEA-15（Ser 104）、磷酸化 PEA-15（Ser 116）减少。PEA-15 的磷酸化影响其抗凋亡功能；PEA-15 磷酸化减少会诱导细胞凋亡。在大脑缺血性损伤期间，EGb 761 预处理维持 PEA-15 磷酸化表明 EGb 761 对大脑缺血性损伤具有神经保护作用。
