Chen Qing, Li Min, Tang Rong-cai, Liu Wei-da, Zhou Wu-qing, Shen Yong-nian, Lv Gui-xia
Jiangsu Province Blood Center, Nanjing, China.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2011 Aug;33(4):371-4. doi: 10.3881/j.issn.1000-503X.2011.04.005.
To investigate whether Candida albicans-native phospholipomannan (PLM) induce an inflammation response through Toll-like receptor(TLRé2 in human acute monocytic leukemia cell line (THP-1) cells.
Human THP-1 monocytes were challenged with PLM in vitro. The mRNA expressions of TLR2, TLR4, proinflammatory cytokine [interleukin(IL)-6], and chemokine (IL-8) were assayed by real time reverse transcription polymerase chain reaction. The secretions of IL-6 and IL-8 were measured by enzyme-linked immunosorbent assay. The expression of TLR2 was analyzed with Western blot.
PLM increased the mRNA expressions and secretions of proinflammatory cytokines (IL-6) and chemokines (IL-8) in THP-1 cells (all P=0.0000). PLM up-regulated the mRNA and protein levels of TLR2 (P=0.0000), whereas the mRNA level of TLR4 was not altered. PLM hydrolyzed with β-D-mannoside manno hydrolase failed to induce gene and protein expressions of TLR2, IL-6, and IL-8. Anti-TLRS-neutralizing antibody blocked the PLM-induced secretions of IL-6 and IL-8 in THP-1 cells (P = 0.0003, P = 0.0010).
Canidada albicans-native PLM may contribute to the inflammatory responses during Candida infection in a TLR2-dependent manner.
研究白色念珠菌天然磷脂甘露聚糖（PLM）是否通过人急性单核细胞白血病细胞系（THP-1）细胞中的Toll样受体（TLR）2诱导炎症反应。
体外用人THP-1单核细胞用PLM进行刺激。通过实时逆转录聚合酶链反应检测TLR2、TLR4、促炎细胞因子[白细胞介素（IL）-6]和趋化因子（IL-8）的mRNA表达。通过酶联免疫吸附测定法测量IL-6和IL-8的分泌。用蛋白质印迹法分析TLR2的表达。
PLM增加了THP-1细胞中促炎细胞因子（IL-6）和趋化因子（IL-8）的mRNA表达和分泌（所有P=0.0000）。PLM上调了TLR2的mRNA和蛋白质水平（P=0.0000），而TLR4的mRNA水平未改变。用β-D-甘露糖苷甘露水解酶水解的PLM未能诱导TLR2、IL-6和IL-8的基因和蛋白质表达。抗TLR2中和抗体阻断了PLM诱导的THP-1细胞中IL-6和IL-8的分泌（P = 0.0003，P = 0.0010）。
白色念珠菌天然PLM可能以TLR2依赖性方式促成念珠菌感染期间的炎症反应。
