Cao Huan, Ji Fang, Wang Xiu-Yu, Shi Jian-Rong
College of Life Science, Nanjing Normal University, Nanjing 210046, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2011 Sep;27(9):975-8.
To synthesize and identify the hapten and complete antigens for Zearalenone for the purpose of preparation of anti-Zearalenone antibodies.
Zearalenone and carboxymethoxylamine hemihydrochloride were incubated to synthesize the hapten ZEN-oxime. The ZEN-oxime was identified by thin layer chromatography (TLC), high performance liquid chromatographic (HPLC) and liquid chromatography-tandem mass spectrometry (LC-MSn). The ZEN-oxime was further conjugated to bovine serum albumin (BSA) or ovalbumin (OVA) to form the complete antigens by NHS ester method. The complete antigens were identified by ultraviolet spectrum, 2, 4, 6-trinitrobenzene sulfonicacid method (TNBS) and immunological analysis.
The results indicated that the hapten and complete antigens were prepared successfully.
The hapten and complete antigens for Zearalenone have been successfully synthesized and identified by several methods, which enables further preparation of anti-Zearalenone antibodies.
合成并鉴定玉米赤霉烯酮的半抗原和完全抗原，用于制备抗玉米赤霉烯酮抗体。
将玉米赤霉烯酮与半胱胺盐酸盐孵育以合成半抗原玉米赤霉烯酮肟。通过薄层色谱法（TLC）、高效液相色谱法（HPLC）和液相色谱-串联质谱法（LC-MSn）对玉米赤霉烯酮肟进行鉴定。通过NHS酯法将玉米赤霉烯酮肟进一步与牛血清白蛋白（BSA）或卵清蛋白（OVA）偶联以形成完全抗原。通过紫外光谱、2,4,6-三硝基苯磺酸法（TNBS）和免疫分析对完全抗原进行鉴定。
结果表明成功制备了半抗原和完全抗原。
已通过多种方法成功合成并鉴定了玉米赤霉烯酮的半抗原和完全抗原，这使得能够进一步制备抗玉米赤霉烯酮抗体。
