Heart Institute (InCor) of the Medical School Hospital, University of São Paulo, São Paulo, Brazil.
Atherosclerosis. 2011 Dec;219(2):532-7. doi: 10.1016/j.atherosclerosis.2011.08.014. Epub 2011 Aug 22.
To evaluate the effects of resistance training (RT) on the metabolism of an LDL-like nanoemulsion and on lipid transfer to HDL, an important step of HDL metabolism.
LDL-like nanoemulsion plasma kinetics was studied in 15 healthy men under regular RT for 1-4 years (age = 25 ± 5 years, VO(2)peak = 50 ± 6 mL/kg/min) and in 15 healthy sedentary men (28 ± 7 years, VO(2)peak = 35 ± 9 mL/kg/min). LDL-like nanoemulsion labeled with (14)C-cholesteryl-ester and (3)H-free-cholesterol was injected intravenously, plasma samples were collected over 24-h to determine decay curves and fractional clearance rates (FCR). Lipid transfer to HDL was determined in vitro by incubating of plasma samples with nanoemulsions (lipid donors) labeled with radioactive free-cholesterol, cholesteryl-ester, triacylglycerols and phospholipids. HDL size, paraoxonase-1 activity and oxidized LDL levels were also determined.
The two groups showed similar LDL and HDL-cholesterol and triacylglycerols, but oxidized LDL was lower in RT (30 ± 9 vs. 61 ± 19 U/L, p = 0.0005). In RT, the nanoemulsion (14)C-cholesteryl-ester was removed twice as fast than in sedentary individuals (FCR: 0.068 ± 0.023 vs. 0.037 ± 0.028, p = 0.002), as well as (3)H-free-cholesterol (0.041 ± 0.025 vs. 0.022 ± 0.023, p = 0.04). While both nanoemulsion labels were removed at the same rate in sedentary individuals, RT (3)H-free-cholesterol was removed slower than (14)C-cholesteryl-ester (p = 0.005). HDL size, paraoxonase 1 and the transfer rates to HDL of the four lipids were the same in both groups.
RT accelerated the clearance of LDL-like nanoemulsion, which probably accounts for the oxidized LDL levels reduction in RT. RT also changed the balance of free and esterified cholesterol FCR's. However, RT had no effect on HDL metabolism related parameters.
评估阻力训练（RT）对 LDL 样纳米乳液代谢以及对 HDL 脂质转移的影响，这是 HDL 代谢的重要步骤。
15 名健康男性在接受 1-4 年常规 RT（年龄=25±5 岁，峰值 VO（2）=50±6 mL/kg/min）和 15 名健康久坐男性（28±7 岁，峰值 VO（2）=35±9 mL/kg/min）下研究 LDL 样纳米乳液的血浆动力学。用（14）C-胆固醇酯和（3）H-游离胆固醇标记 LDL 样纳米乳液，静脉注射后在 24 小时内收集血浆样本以确定衰减曲线和分数清除率（FCR）。通过孵育标记有放射性游离胆固醇、胆固醇酯、三酰甘油和磷脂的纳米乳液，在体外测定脂质向 HDL 的转移。还测定了 HDL 大小、对氧磷酶-1 活性和氧化型 LDL 水平。
两组的 LDL 和 HDL-胆固醇和三酰甘油相似，但 RT 中的氧化型 LDL 水平较低（30±9 与 61±19 U/L，p=0.0005）。在 RT 中，纳米乳液（14）C-胆固醇酯的清除速度是久坐个体的两倍（FCR：0.068±0.023 与 0.037±0.028，p=0.002），（3）H-游离胆固醇也是如此（0.041±0.025 与 0.022±0.023，p=0.04）。虽然在久坐个体中两种纳米乳液标记物的清除速度相同，但 RT 中的（3）H-游离胆固醇的清除速度比（14）C-胆固醇酯慢（p=0.005）。两组的 HDL 大小、对氧磷酶 1 和四种脂质向 HDL 的转移率相同。
RT 加速了 LDL 样纳米乳液的清除，这可能是 RT 中氧化型 LDL 水平降低的原因。RT 还改变了游离和酯化胆固醇 FCR 的平衡。然而，RT 对与 HDL 代谢相关的参数没有影响。
