Exercise and Metabolic Disease Research Laboratory, Translational Sciences Section, School of Nursing, University of California, Los Angeles, Los Angeles, California.
J Appl Physiol (1985). 2013 Oct 1;115(7):1043-9. doi: 10.1152/japplphysiol.00359.2013. Epub 2013 Jul 25.
We examined the impact of strength fitness and body weight on the redox properties of high-density lipoprotein (HDL) and associations with indices of vascular and metabolic health. Ninety young men were categorized into three groups: 1) overweight untrained (OU; n = 30; BMI 30.7 ± 2.1 kg/m(2)); 2) overweight trained [OT; n = 30; BMI 29.0 ± 1.9; ≥4 d/wk resistance training (RT)]; and 3) lean trained (LT; n = 30; BMI 23.7 ± 1.4; ≥4 d/wk RT). Using a novel assay on the basis of the HDL-mediated rate of oxidation of dihydrorhodamine (DOR), we determined the functional (redox) properties of HDL and examined correlations between DOR and indices of vascular and metabolic health in the cohort. DOR was significantly lower in both trained groups compared with the untrained group (LT, 1.04 ± 0.49; OT, 1.39 ± 0.57; OU, 1.80 ± 0.74; LT vs. OU P < 0.00001; OT vs. OU P = 0.02), however, DOR in the OT group was not significantly different from that of the LT group. DOR was negatively associated with HDL-cholesterol (R = -0.64), relative strength (R = -0.42), sex hormone-binding globulin (R = -0.42), and testosterone (R = -0.35) (all P ≤ 0.001); whereas DOR was positively associated with triglycerides (R = 0.39, P = 0.002), oxidized low-density lipoprotein (R = 0.32), body mass index (R = 0.43), total mass (R = 0.35), total fat mass (R = 0.42), waist circumference (R = 0.45), and trunk fat mass (R = 0.42) (all P ≤ 0.001). Chronic RT is associated with improved HDL redox activity. This may contribute to the beneficial effects of RT on reducing cardiovascular disease risk, irrespective of body weight status.
我们研究了力量健身和体重对高密度脂蛋白（HDL）的氧化还原特性的影响，以及与血管和代谢健康指标的关联。90 名年轻男性分为三组：1）超重未训练组（OU；n=30；BMI 30.7±2.1kg/m²）；2）超重训练组（OT；n=30；BMI 29.0±1.9；每周≥4 天进行抗阻训练（RT））；3）瘦训练组（LT；n=30；BMI 23.7±1.4；每周≥4 天进行 RT）。我们基于 HDL 介导的二氢罗丹明（DOR）氧化速率的新测定方法，测定了 HDL 的功能（氧化还原）特性，并在队列中检查了 DOR 与血管和代谢健康指标之间的相关性。与未训练组相比，两组训练组的 DOR 均显著降低（LT：1.04±0.49；OT：1.39±0.57；OU：1.80±0.74；LT 与 OU 比较，P<0.00001；OT 与 OU 比较，P=0.02），然而，OT 组的 DOR 与 LT 组的 DOR 无显著差异。DOR 与 HDL-胆固醇（R=-0.64）、相对力量（R=-0.42）、性激素结合球蛋白（R=-0.42）和睾酮（R=-0.35）呈负相关（均 P≤0.001）；而 DOR 与甘油三酯（R=0.39，P=0.002）、氧化型低密度脂蛋白（R=0.32）、体重指数（R=0.43）、总质量（R=0.35）、总脂肪质量（R=0.42）、腰围（R=0.45）和躯干脂肪质量（R=0.42）呈正相关（均 P≤0.001）。慢性 RT 与改善 HDL 氧化还原活性有关。这可能有助于 RT 降低心血管疾病风险的有益作用，而与体重状况无关。
