Siatkowski R Michael, Yanovitch Tammy L, Ash John D, Moreau Annie
Dean McGee Eye Institute, Edmond, Oklahoma, USA.
J AAPOS. 2011 Aug;15(4):370-3. doi: 10.1016/j.jaapos.2011.04.005.
To determine the effect of intraperitoneal and intravitreal D-penicillamine (DPA) on retinal neovascularization in a murine model of oxygen-induced retinopathy.
On postnatal day 7, 16 mice were injected intraperitoneally with 300 mg/kg/day DPA for 3 days followed by 50 mg/kg/day for 7 days. A different group of 7 mice were injected intraperitoneally with 600 mg/kg/day DPA for 3 days followed by 100 mg/kg/day for 7 days. A third group of 14 mice were injected with 1,500 mg/kg/day DPA for 2 days; a control cohort of 17 mice received intraperitoneal phosphate-buffered saline (PBS). An additional 15 mice underwent intravitreal injection of 1 μL of 100 mg/mL DPA in the right eye and 1 μL PBS intravitreally in the left eye as a control. All groups were placed in a 75% oxygen chamber for 7 days then room air for 3 days before being sacrificed and enucleated. The retinas were stained and flat-mounted to determine the severity of retinal neovascularization by quantifying neovascular buds.
After intraperitoneal injection, the mean number of glomeruli and tubules was similar in the DPA and PBS groups (P = 1.0), regardless of DPA dosage. The dosage of 1,500 mg/kg/day proved to be uniformly lethal. After intravitreal injections, the mean number of glomeruli (P = 0.16) and tubules (P = 0.7) were similar in the DPA and PBS groups.
Neither intraperitoneal nor intravitreal injection of DPA inhibits retinal neovascularization in a murine model of oxygen-induced retinopathy.
确定腹腔内和玻璃体内注射D-青霉胺（DPA）对氧诱导性视网膜病变小鼠模型视网膜新生血管形成的影响。
出生后第7天，16只小鼠腹腔内注射300mg/kg/天的DPA，持续3天，随后7天注射50mg/kg/天。另一组7只小鼠腹腔内注射600mg/kg/天的DPA，持续3天，随后7天注射100mg/kg/天。第三组14只小鼠注射1500mg/kg/天的DPA，持续2天；17只小鼠的对照组腹腔内注射磷酸盐缓冲盐水（PBS）。另外15只小鼠右眼玻璃体内注射1μL 100mg/mL的DPA，左眼玻璃体内注射1μL PBS作为对照。所有组在75%氧气舱中放置7天，然后在空气中放置3天，之后处死并摘除眼球。对视网膜进行染色并平铺，通过量化新生血管芽来确定视网膜新生血管形成的严重程度。
腹腔注射后，无论DPA剂量如何，DPA组和PBS组的肾小球和肾小管平均数量相似（P = 1.0）。1500mg/kg/天的剂量被证明具有一致的致死性。玻璃体内注射后，DPA组和PBS组的肾小球平均数量（P = 0.16）和肾小管平均数量（P = 0.7）相似。
在氧诱导性视网膜病变小鼠模型中，腹腔内或玻璃体内注射DPA均不能抑制视网膜新生血管形成。
