College of Chemistry, Jilin University, Changchun, China.
J Chromatogr A. 2011 Oct 14;1218(41):7254-63. doi: 10.1016/j.chroma.2011.08.063. Epub 2011 Aug 25.
Ionic liquid-based homogeneous liquid-liquid microextraction (IL-based HLLME) high-performance liquid chromatography was developed and applied to the extraction, separation and determination of some antibiotics in milk. The proteins and lipids were removed by adding salt and adjusting the pH value. The homogeneous extraction was applied to the improvement of recoveries for IL phase and analytes. The experimental parameters of the IL-based HLLME, including salt concentration in sample solution, pH value of sample solution, volume of [C(6)MIM][BF(4)], amount of ion-pairing agent (NH(4)PF(6)), and extraction time, were evaluated. The limits of detection for enoxacin, pefloxacin, norfloxacin, enrofloxacin, sulfamethoxazole and sulfadimethoxine were 15.8, 7.07, 5.13, 4.00, 7.79 and 8.33 μg L(-1), respectively. When the proposed method was applied to the analysis of milk samples the recoveries of the analytes ranged from 92.5 to 118.6% and relative standard deviations were lower than 7.00%.
基于离子液体的均相液-液微萃取（IL 基 HLLME）高效液相色谱法被开发并应用于牛奶中一些抗生素的提取、分离和测定。通过添加盐和调节 pH 值去除蛋白质和脂质。均相萃取用于提高 IL 相和分析物的回收率。对 IL 基 HLLME 的实验参数进行了评价，包括样品溶液中的盐浓度、样品溶液的 pH 值、[C（6）MIM][BF（4）]的体积、离子对试剂（NH（4）PF（6））的量和萃取时间。恩诺沙星、培氟沙星、诺氟沙星、恩氟沙星、磺胺甲恶唑和磺胺二甲嘧啶的检测限分别为 15.8、7.07、5.13、4.00、7.79 和 8.33 μg L（-1）。当将该方法应用于牛奶样品的分析时，分析物的回收率在 92.5%至 118.6%之间，相对标准偏差低于 7.00%。
