离子液体分散微萃取与高效液相色谱-质谱联用检测水中防污生物杀灭剂

Ionic Liquid-Dispersive Micro-Extraction and Detection by High Performance Liquid Chromatography-Mass Spectrometry for Antifouling Biocides in Water.

机构信息

College of Science, China Agricultural University, Beijing 100193, China.

Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China.

出版信息

Molecules. 2023 Jan 28;28(3):1263. doi: 10.3390/molecules28031263.

A simple analytical method was developed and evaluated for the determination of two antifouling biocides using an ionic liquid-dispersive liquid-liquid micro-extraction (IL-DLLME) and a high-performance liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS) analysis. Irgarol 1051 and Sea-Nine 211 were extracted from deionized water, lake water, and seawater using IL 1-hexyl-3-methylimidazolium hexafluorophosphate ([HMIm][PF6]) and ethyl acetate as the extraction solvent and the dispersion solvent. Several factors were considered, including the type and volume of extraction and dispersive solvent, IL amount, sample pH, salt effect, and cooling temperature. The developed method resulted in a recovery range of 78.7-90.3%, with a relative standard deviation (RSD, = 3) less than 7.5%. The analytes were enriched greater than 40-fold, and the limits of detection (LOD) for two antifouling biocides were 0.01-0.1 μg L. The method was effectively applied for the analysis of real samples of freshwater as well as samples of seawater.

开发并评价了一种简单的分析方法，用于使用离子液体分散液液微萃取（IL-DLLME）和高效液相色谱-电喷雾电离质谱（LC-ESI-MS）分析来测定两种防污生物杀灭剂。使用 IL 1-己基-3-甲基咪唑六氟磷酸盐([HMIm][PF6])和乙酸乙酯作为萃取溶剂和分散溶剂，从去离子水、湖水和海水中萃取了 Irgarol 1051 和 Sea-Nine 211。考虑了多种因素，包括萃取和分散溶剂的类型和体积、IL 用量、样品 pH 值、盐效应和冷却温度。所开发的方法的回收率范围为 78.7-90.3%，相对标准偏差（RSD，n = 3）小于 7.5%。分析物的富集倍数大于 40 倍，两种防污生物杀灭剂的检出限（LOD）为 0.01-0.1 μg L。该方法有效地应用于淡水和海水实际样品的分析。