Department of Health Laboratory Technology, College of Public Health, China Medical University, No. 92 of Beima Road, Heping District, Shenyang 110001, PR China.
J Pharm Biomed Anal. 2012 Jan 5;57:120-4. doi: 10.1016/j.jpba.2011.08.032. Epub 2011 Aug 26.
Realgar is a traditional Chinese medicine, which has been used for thousands of years and are claimed to have therapeutic effects. The toxicity from realgar or realgar-containing traditional medicines has raised public concern. However, the neurotoxicity induced by realgar is less reported. Amino acid neurotransmitters are closely linked to the vulnerability of the immature brain to neuronal injury. The investigation of amino acid neurotransmitters is important to understand the evolution of developmental brain damage. An improved HPLC-UV method was developed and applied to analyzing amino acid neurotransmitters of aspartate, glutamate, glutamine, homocysteine, serine, glycine, γ-aminobutyric acid and taurine in brain tissues of immature rats after the treatment of realgar. Significant changes of these amino acid neurotransmitters were observed in realgar treated groups. Negative correlations were found between the levels of some amino acids and the contents of arsenic in brain tissues. The result indicates that the neurotoxicity induced by realgar is associated with its effects on amino acid neurotransmitters.
雄黄是一种传统中药，已使用了数千年，据称具有治疗效果。雄黄或含雄黄的传统药物的毒性引起了公众的关注。然而，雄黄引起的神经毒性报道较少。氨基酸神经递质与未成熟大脑对神经元损伤的易感性密切相关。研究氨基酸神经递质对于了解发育性脑损伤的演变很重要。建立了一种改进的 HPLC-UV 方法，并应用于分析雄黄处理后的未成熟大鼠脑组织中天冬氨酸、谷氨酸、谷氨酰胺、同型半胱氨酸、丝氨酸、甘氨酸、γ-氨基丁酸和牛磺酸等氨基酸神经递质。在雄黄处理组中观察到这些氨基酸神经递质的显著变化。一些氨基酸的水平与脑组织中砷的含量之间存在负相关。结果表明，雄黄引起的神经毒性与其对氨基酸神经递质的作用有关。
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