[亚慢性雄黄暴露对幼鼠脑内谷氨酸和谷氨酰胺的影响]

[Effect of subchronic realgar exposure on Glu and Gln in infant rat brain].

作者信息

Chang Bei, Huo Tao-Guang, Zhang Ying-Hua, Li Wei-Kai, Yang Hui-Lei, Jiang Hong, Sun Gui-Fan

机构信息

College of Public Health, China Medical University, Shenyang 110001, China.

出版信息

Zhong Yao Cai. 2012 Nov;35(11):1817-20.

PMID:23627097

Abstract

OBJECTIVE

To study the effect of realgar on Glu and Gln on rat brain tissues.

METHODS

Forty-eight Wistar rats were divided into 4 groups randomly:control group,low dosage group, moderate dosage group and high dosage group. The treatment groups were treated with realgar by gastric perfusion at a dosage of 0.3 g/kg, 0.9 g/kg, 2.7 g/kg and the control group ones were orally given the same volume of 0.5% sodium carboxymethylcellulose (CMC-Na) for 6 weeks. The contents of inorganic arsenic, monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA) in brain tissues were measured by hydride generation-atomic absorption (HG-AAS) method. The contents of amino acid neurotransmitters in brain tissues of rats were determined by means of high performance liquid chromatography with precolumn derivatization.

RESULTS

The levels of MMA and DMA in brain increased as the dosage of realgar increased, while the second methylation index declined. Compared with control group,the levels of Glu was significantly decreased in realgar treated group (P < 0.05); Gln also tended to decrease and that of low dosage group was obviously decreased compared with controls.

CONCLUSION

Realgar exposure can cause accumulation of MMA and DMA,declination of second methylation index and the reduction of Glu and Gln in brain tissue.

摘要

目的

研究雄黄对大鼠脑组织中谷氨酸（Glu）和谷氨酰胺（Gln）的影响。

方法

将48只Wistar大鼠随机分为4组：对照组、低剂量组、中剂量组和高剂量组。治疗组分别以0.3 g/kg、0.9 g/kg、2.7 g/kg的剂量通过灌胃给予雄黄，对照组给予等体积的0.5%羧甲基纤维素钠（CMC-Na），连续6周。采用氢化物发生-原子吸收光谱法（HG-AAS）测定脑组织中无机砷、一甲基砷酸（MMA）和二甲基砷酸（DMA）的含量。采用柱前衍生高效液相色谱法测定大鼠脑组织中氨基酸类神经递质的含量。