Department of Internal Medicine and WHO Collaborating Center on Viral Hepatitis, College of Medicine, The Catholic University of Korea, 137-040 Seoul, Republic of Korea.
J Clin Virol. 2011 Dec;52(4):363-6. doi: 10.1016/j.jcv.2011.08.006. Epub 2011 Sep 9.
Single nucleotide polymorphisms (SNPs) near the IL28B gene have recently been described as predictors of antiviral therapy responses in patients with hepatitis C virus (HCV) genotype-1.
The aim of this study was to investigate the association between genetic variation near the IL28B gene and treatment outcome prediction in Korean patients receiving peg-interferon (PEG-IFN) plus ribavirin therapy.
The allelic discrimination assay by Taqman real-time PCR was developed to determine genotypes of SNPs, rs12979860 and rs8099917, which were analyzed in 65 Korean patients with HCV genotype-1.
For rs12979860, the frequency of patients with sustained virological response (SVR) was 70.2% in those with the CC genotype and 25% in those with the CT genotype. Early virological response (EVR) in patients with the CC genotype (84.2%) was higher than in those with the CT genotype (25.0%). For rs8099917, patients with the TT genotype showed significantly higher in SVR and EVR than those with the TG/GG genotype (69.6% vs 33.3% and 82.1% vs 44.4%, respectively). With regards to the genotype frequency of the SNPs, the homozygous genotypes for rs12979860 (CC) or rs8099917 (TT) in Korean patients showed a significantly higher frequency as compared with other ethnicities; Caucasians, African-American, Hispanic, and Japanese.
These results demonstrate that the genotypes rs12979860 CC and rs8099917 TT were more frequently observed in Korean patients compared to other ethnicities, and suggest that the genetic characteristics of patients may be prognostic factor that predicts antiviral response to PEG-IFN therapy for chronic hepatitis C.
最近发现，白细胞介素 28B 基因附近的单核苷酸多态性（SNPs）可预测丙型肝炎病毒（HCV）基因型 1 患者的抗病毒治疗反应。
本研究旨在探讨韩国接受聚乙二醇干扰素（PEG-IFN）加利巴韦林治疗的患者中，白细胞介素 28B 基因附近遗传变异与治疗结果预测之间的关系。
采用 Taqman 实时 PCR 等位基因鉴别检测法检测 65 例 HCV 基因型 1 韩国患者的 SNP（rs12979860 和 rs8099917）基因型。
对于 rs12979860，CC 基因型患者的持续病毒学应答（SVR）率为 70.2%，CT 基因型患者为 25%。CC 基因型患者的早期病毒学应答（EVR）（84.2%）高于 CT 基因型患者（25.0%）。对于 rs8099917，TT 基因型患者的 SVR 和 EVR 显著高于 TG/GG 基因型患者（69.6%比 33.3%和 82.1%比 44.4%）。就 SNP 的基因型频率而言，韩国患者 rs12979860（CC）或 rs8099917（TT）的纯合基因型与其他种族相比频率更高；白种人、非裔美国人、西班牙裔和日本人。
这些结果表明，与其他种族相比，韩国患者中 rs12979860 CC 和 rs8099917 TT 基因型更为常见，这表明患者的遗传特征可能是预测慢性丙型肝炎对 PEG-IFN 治疗的抗病毒反应的预后因素。
