Genetic and Reproductive Toxicology Group, Division of Biomedical Sciences, University of Bradford, Bradford BD7 1DP, UK.
Food Chem Toxicol. 2012 Feb;50(2):124-9. doi: 10.1016/j.fct.2011.08.020. Epub 2011 Sep 1.
The food mutagens IQ (2-amino-3-methylimidazo[4,5-f]quinoline) and PhIP (2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine) are heterocyclic amines (HCA), generated when heating proteinaceous food. This study investigates the protective potential of the flavonoids quercetin (Q) and rutin (R) against oxidative stress induced in vitro by IQ and PhIP in lymphocytes from healthy individuals and untreated, newly diagnosed colon cancer patients using the Comet assay. In the presence of up to 500μM Q and R, the DNA damage resulting from a high dose of PhIP (75μM) or IQ (150μM) was significantly reduced (P<0.001) to levels comparable to six times lower IQ or 7.5 times lower PhIP doses. Lymphocytes from colon cancer patients had greater baseline DNA damage than those from healthy individuals (P<0.01) and this higher level of damage was also observed throughout in vitro treatment. Except for the >50years of age group and male gender, confounding factors such as smoking, drinking and/or dietary habits were not found to be significant. In conclusion, flavonoids reduced oxidative stress caused by food mutagens in vitro in lymphocytes of healthy individuals and colon cancer patients. Thus, dietary supplementation with flavonoid-rich vegetables and fruits may prove very effective in protecting against oxidative stress.
食品致突变剂 IQ（2-氨基-3-甲基咪唑[4,5-f]喹啉）和 PhIP（2-氨基-1-甲基-6-苯基咪唑[4,5-b]吡啶）是杂环胺（HCA），当加热蛋白质食物时会产生。本研究使用彗星试验调查了类黄酮槲皮素（Q）和芦丁（R）对健康个体和未经治疗的新诊断结肠癌患者淋巴细胞中由 IQ 和 PhIP 体外诱导的氧化应激的保护潜力。在高达 500μM 的 Q 和 R 存在下，由高剂量 PhIP（75μM）或 IQ（150μM）引起的 DNA 损伤显著降低（P<0.001），降低到相当于 IQ 剂量低 6 倍或 PhIP 剂量低 7.5 倍的水平。与健康个体相比，结肠癌患者的淋巴细胞的基线 DNA 损伤更大（P<0.01），并且在整个体外治疗过程中也观察到这种更高水平的损伤。除了年龄大于 50 岁和男性性别之外，没有发现吸烟、饮酒和/或饮食习惯等混杂因素具有显著影响。总之，类黄酮可减少健康个体和结肠癌患者淋巴细胞中由食物致突变剂引起的体外氧化应激。因此，富含类黄酮的蔬菜和水果的饮食补充可能被证明对预防氧化应激非常有效。
