Istituto per la Protezione delle Piante CNR, Via Università 133, 80055 Portici (NA), Italy.
J Plant Physiol. 2011 Dec 15;168(18):2225-33. doi: 10.1016/j.jplph.2011.07.008. Epub 2011 Sep 9.
Pleiotropic drug resistant (PDR/ABCG) genes are involved in plant response to biotic and abiotic stresses. In this work, we cloned, from Solanum tuberosum, four PDR/ABCG transporter genes named StPDR1, StPDR2, StPDR3 and StPDR4, which were differentially expressed in plant tissues and cell cultures. A number of different chemically unrelated compounds were found to regulate the transcript levels of the four genes in cultured cells. In particular, StPDR2 was highly up-regulated in the presence of Botrytis cinerea cell walls, NaCl, 2,4-dichlorophenol, sclareol and α-solanin and biological compounds. The expression of the genes was also investigated by real time RT-PCR during infection by Phytophthora infestans. StPDR1 and StPDR2 were up-regulated about 13- and 37-fold at 48 h post-infection (hpi), StPDR3 was expressed (4-5-fold) at 24 and 48 hpi and then rapidly decreased, while StPDR4 RNA accumulation was stimulated (about 4-fold) at 12 and 24 hpi, decreased at 48 hpi and increased again at 96 hpi. We discuss the role of StPDR1-4 genes in response to pathogens and abiotic stresses.
多药耐药（PDR/ABCG）基因参与植物对生物和非生物胁迫的反应。在这项工作中，我们从马铃薯中克隆了四个 PDR/ABCG 转运蛋白基因，分别命名为 StPDR1、StPDR2、StPDR3 和 StPDR4，它们在植物组织和细胞培养中差异表达。许多不同的化学上无关的化合物被发现可调节培养细胞中这四个基因的转录水平。特别是，StPDR2 在存在 Botrytis cinerea 细胞壁、NaCl、2,4-二氯苯酚、sclareol 和α-茄碱和生物化合物时高度上调。通过实时 RT-PCR 还研究了基因在感染 Phytophthora infestans 期间的表达。StPDR1 和 StPDR2 在感染后 48 小时（hpi）上调约 13 倍和 37 倍，StPDR3 在 24 和 48 hpi 时表达（4-5 倍），然后迅速下降，而 StPDR4 的 RNA 积累在 12 和 24 hpi 时受到刺激（约 4 倍），在 48 hpi 时下降，并在 96 hpi 时再次增加。我们讨论了 StPDR1-4 基因在应对病原体和非生物胁迫中的作用。
