Woldemeskel Moges, Liggett Alan, Ilha Marcia, Saliki Jeremiah T, Johnson Leslie P
The University of Georgia, College of Veterinary Medicine, Department of Pathology, Tifton Veterinary Diagnostic and Investigational Laboratory, 43 Brighton Road, Tifton, GA 31793, USA.
J Vet Diagn Invest. 2011 May;23(3):576-80. doi: 10.1177/1040638711403429.
Erythema multiforme (EM) was diagnosed in a litter of English Setter puppies. The puppies developed erythematous cutaneous lesions at the age of 2 weeks. Microscopically, there was individual keratinocyte apoptosis associated with lymphocyte exocytosis in all layers of the epidermis. Intranuclear viral inclusions were seen in multiple tissues and organs. Tissues from the tongue, lymph node, spleen, skin, and small intestine were positive for Canine parvovirus-2 (CPV-2) and negative for Canine distemper virus (CDV) and Canid herpesvirus 1 by fluorescent antibody test. Negative-staining electron microscopy detected parvovirus particles in the intestinal contents. The skin and small intestine were positive for CPV-2b and negative for CDV by polymerase chain reaction. The mucocutaneous junctions and small intestines stained positive for CPV by immunohistochemistry. The present report documents CPV-2b-associated EM in a litter of English Setters and substantiates the single previous report associating EM with CPV-2. The finding suggests that CPV should be considered as a possible cause of EM in dogs.
一窝英国塞特犬幼犬被诊断出患有多形红斑（EM）。这些幼犬在2周龄时出现了红斑性皮肤病变。显微镜下，在表皮各层均可见到与淋巴细胞外渗相关的单个角质形成细胞凋亡。在多个组织和器官中可见核内病毒包涵体。通过荧光抗体试验，舌、淋巴结、脾脏、皮肤和小肠组织对犬细小病毒2型（CPV-2）呈阳性，对犬瘟热病毒（CDV）和犬疱疹病毒1呈阴性。负染电子显微镜在肠道内容物中检测到细小病毒颗粒。通过聚合酶链反应，皮肤和小肠对CPV-2b呈阳性，对CDV呈阴性。免疫组织化学显示，皮肤黏膜交界处和小肠对CPV呈阳性。本报告记录了一窝英国塞特犬中与CPV-2b相关的EM，并证实了之前关于EM与CPV-2相关的唯一一份报告。这一发现表明，CPV应被视为犬EM的一个可能病因。
