Southern Community Laboratories Ltd., Dunedin, New Zealand.
Invest Ophthalmol Vis Sci. 2011 Oct 21;52(11):8300-9. doi: 10.1167/iovs.11-7772.
PURPOSE. To report the ultrastructure of cells and extracellular matrix components in Molteno implant capsules examined by scanning and transmission electron microscopy.
Ultrastructural features including cytology, distribution of apoptotic cells, collagens, basement membranes, elastic fibrils, and glycoproteins were examined by scanning and transmission electron microscopy. Findings were correlated with the clinical features of 31 specimens of glaucomatous eyes treated with Molteno implants 0.3 to 14.9 years previously.
Capsules showed two layers: an outer, moderately cellular vascular layer of normal-appearing cells and collagen and an inner, avascular, hypocellular layer of altered cells and collagen. Cells included fibroblasts, myofibroblasts, and tissue histiocytes that showed features indicating metabolic activity, with swelling, vacuolation, and apoptosis, and the formation of numerous membrane-bound vesicles. These features, together with alteration and disintegration of extracellular matrix, increased with time after surgery.
The results support those in previous light microscopic studies and indicate that the normal life cycle of capsules in both primary and secondary glaucoma include continual outer surface renewal balanced by inner surface degeneration associated with apoptosis and breakdown of tissue matrix components which become more marked over time.
目的。报告用扫描和透射电子显微镜检查莫尔顿植入物胶囊中的细胞和细胞外基质成分的超微结构。
用扫描和透射电子显微镜检查细胞学、凋亡细胞、胶原、基底膜、弹性纤维和糖蛋白的分布等超微结构特征。将发现与 31 例用莫尔顿植入物治疗的青光眼眼标本的临床特征相关联，这些标本在 0.3 至 14.9 年前接受了治疗。
胶囊显示出两层：外层为正常外观的细胞和胶原组成的中等细胞血管层，内层为无血管的、细胞稀少的胶原层。细胞包括成纤维细胞、肌成纤维细胞和组织组织细胞，它们显示出代谢活性的特征，表现为肿胀、空泡化和细胞凋亡，以及形成大量膜结合囊泡。这些特征与细胞外基质的改变和崩解一起，随着手术后时间的推移而增加。
结果支持以前的光镜研究结果，并表明原发性和继发性青光眼的囊正常生命周期包括持续的外表面更新，由与细胞凋亡和组织基质成分分解相关的内表面退化平衡，随着时间的推移，这些变化变得更加明显。
