Microbial Sciences Division, MACS-Agharkar Research Institute, Pune 411004, Maharashtra, India.
Appl Biochem Biotechnol. 2011 Nov;165(5-6):1406-13. doi: 10.1007/s12010-011-9356-2. Epub 2011 Sep 10.
Production of the fibrinolytic enzyme was carried out using 2.5-L glass fermentor, culture of thermophilic Streptomyces sp., and glucose yeast extract peptone medium of pH 8.0. Five successive batches were carried out under controlled fermentation conditions viz., agitation 140 rpm, aeration 0.5 vvm, 55 °C, and 18 h. The total protein extracellularly produced in the cell-free broth was ~300-500 mg/L. The enzyme belongs to serine endopeptidase type. Studies on the fibrin degradation indicate that the enzyme degrades the fibrin into small molecular weight products as seen from HPLC profile. Phase-contrast microscopic structure of fibrin showed that enzyme cleaves the fibrin filaments. The ex vivo activity of the actinokinase was compared with 500 IU of urokinase and 350 IU of streptokinase. The ex vivo clot lysis was found to be faster as compared to the commercial available enzymes.
采用 2.5L 玻璃发酵罐、嗜热链霉菌培养物和 pH8.0 的葡萄糖酵母提取物蛋白胨培养基进行纤维蛋白溶酶的生产。在控制发酵条件下进行了五批连续发酵，搅拌速度为 140rpm，通气量为 0.5vvm，温度为 55°C，时间为 18 小时。无细胞发酵液中分泌的总蛋白约为 300-500mg/L。该酶属于丝氨酸内肽酶。纤维蛋白降解研究表明，该酶将纤维蛋白降解为小分子产物，从 HPLC 图谱可以看出。纤维蛋白的相差显微镜结构显示酶可切割纤维蛋白丝。纤维蛋白溶酶的体外活性与 500IU 的尿激酶和 350IU 的链激酶进行了比较。与市售酶相比，体外凝块溶解更快。
