De Calisto Jaime, Villablanca Eduardo J, Wang Sen, Bono Maria R, Rosemblatt Mario, Mora J Rodrigo
Gastrointestinal Unit, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
Methods Mol Biol. 2012;757:411-34. doi: 10.1007/978-1-61779-166-6_24.
Effector/memory T cells can migrate to most extra-lymphoid tissues in the body. However, migration to the intestinal mucosa requires the expression of very specific homing receptors on T cells, integrin α4β7 and chemokine receptor CCR9. These receptors are induced by all-trans retinoic acid (RA), a vitamin A metabolite that is specifically synthesized by gut-associated dendritic cells (DC), but not by extra-intestinal DC. Here we summarize some general concepts on T cell homing with an emphasis on the gut mucosa. We also discuss experimental strategies to generate gut-homing T cells in vivo and in vitro and the techniques to track gut-homing T cells.
效应/记忆T细胞可迁移至体内大多数淋巴外组织。然而，迁移至肠道黏膜需要T细胞表达非常特异的归巢受体，即整合素α4β7和趋化因子受体CCR9。这些受体由全反式视黄酸（RA）诱导产生，RA是一种维生素A代谢产物，由肠道相关树突状细胞（DC）特异性合成，而非肠道外DC。在此，我们总结一些关于T细胞归巢的一般概念，重点是肠道黏膜。我们还讨论了在体内和体外产生肠道归巢T细胞的实验策略以及追踪肠道归巢T细胞的技术。
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