National Research Institute of Police Science, Kashiwa, Chiba, Japan.
Rapid Commun Mass Spectrom. 2011 Sep 15;25(17):2397-406. doi: 10.1002/rcm.5145.
Amphetamine-type stimulants (ATS) such as methamphetamine are widely abused and can cause toxic effects in the body. In this study, a simple and accurate analytical method for distribution measurement of drugs in organs was developed to visualize localization of ATS in organs and to complement drug distribution by mass spectrometry imaging (MSI). The brain, liver and kidney from rats to which ATS had been administered were segmented into blocks of 2×2×2 mm3 at -30°C. Each organ block was micropulverized with a stainless-steel bullet at -80°C. The concentrations of drugs in each block were measured by liquid chromatography/tandem mass spectrometry. The three-dimensional distribution of drugs in a whole organ was expressed using color gradation of drug concentration after reconstruction of all blocks to the original locations. The distribution was also compared with that obtained by MSI. This method enabled measurement of drug distribution in organs with simple and clean procedures and accurate quantification unlike autoradiography and MSI. The methamphetamine concentrations were different between parts in an organ, particularly in the kidney. This method could be applicable to the measurement of the distribution of compounds in various solid samples and could be used as a complementary method for the measurement of the distribution of compounds by MSI.
苯丙胺类兴奋剂（ATS），如甲基苯丙胺，被广泛滥用，并可能在体内造成毒性作用。在这项研究中，开发了一种简单而准确的分析方法，用于测量器官中药物的分布，以可视化 ATS 在器官中的定位，并通过质谱成像（MSI）补充药物分布。将给予 ATS 的大鼠的大脑、肝脏和肾脏在-30°C 下分割成 2×2×2mm3 的块。每个器官块在-80°C 下用不锈钢子弹微粉碎。通过液相色谱/串联质谱法测量每个块中药物的浓度。通过将所有块重建到原始位置后，使用药物浓度的颜色渐变来表示整个器官中药物的三维分布。还将分布与 MSI 获得的分布进行了比较。与放射自显影和 MSI 相比，该方法能够以简单、清洁的程序测量器官中的药物分布，并进行准确的定量。在器官的不同部位，特别是肾脏，甲基苯丙胺的浓度不同。该方法可适用于各种固体样品中化合物分布的测量，并可作为 MSI 测量化合物分布的补充方法。
