Spencer S C, Fabre J W
Blond McIndoe Centre, Queen Victoria Hospital, East Grinstead, Sussex, United Kingdom.
J Exp Med. 1990 Jun 1;171(6):1841-51. doi: 10.1084/jem.171.6.1841.
Immunohistological studies with a mouse anti-rat macrophage mAb (BMAC-5) demonstrated the presence of numerous positive cells in the interstitial connective tissues of many organs. The pattern resembled that seen with anti-MHC class II antibodies, with the striking exception that BMAC-5+ cells were rare or absent in the portal triad, the islets of Langerhans, and the kidney. Double-labeling fluorescence studies were therefore performed in rat heart using the BMAC-5 mAb in combination with rabbit antisera to pure rat class II MHC antigens and pure rat leukocyte common (CD45) antigens. The tissue macrophages in heart were identified as BMAC-5+, MHC class II-negative, leukocyte common antigen-positive cells. They could be distinguished from the BMAC-5-, MHC class II-positive, leukocyte common antigen-positive interstitial dendritic cells. Moreover, 7 d after lethal irradiation, the class II-positive interstitial dendritic cells had completely disappeared from heart, whereas the BMAC-5+ macrophages were present in undiminished numbers. These studies strongly suggest that the interstitial dendritic cell and the tissue macrophage represent two distinct populations of leukocytes within the connective tissues of antigenically secluded organs such as the heart. They have potentially important implications for the physiology of the immune system, as well as for autoimmunity and transplantation.
用小鼠抗大鼠巨噬细胞单克隆抗体（BMAC-5）进行的免疫组织学研究表明，许多器官的间质结缔组织中存在大量阳性细胞。这种模式与抗MHC II类抗体所见相似，但显著不同的是，在门脉三联体、胰岛和肾脏中，BMAC-5+细胞很少或不存在。因此，在大鼠心脏中进行了双标记荧光研究，使用BMAC-5单克隆抗体与兔抗血清结合，以检测纯大鼠II类MHC抗原和纯大鼠白细胞共同抗原（CD45）。心脏中的组织巨噬细胞被鉴定为BMAC-5+、MHC II类阴性、白细胞共同抗原阳性细胞。它们可以与BMAC-5-、MHC II类阳性、白细胞共同抗原阳性的间质树突状细胞区分开来。此外，致死性照射7天后，II类阳性间质树突状细胞已从心脏完全消失，而BMAC-5+巨噬细胞数量未减少。这些研究强烈表明，间质树突状细胞和组织巨噬细胞代表了抗原隔离器官（如心脏）结缔组织内两种不同的白细胞群体。它们对免疫系统的生理学以及自身免疫和移植具有潜在的重要意义。
