Hart D N, Fabre J W
J Exp Med. 1981 Aug 1;154(2):347-61. doi: 10.1084/jem.154.2.347.
In this study, we have used a mouse monoclonal antibody to rat Ia (RT1-B or class II) antigens to demonstrate, by immunofluorescence on frozen sections, intensely Ia-positive dendritic cells in the interstitial connective tissues of every tissue we have examined (heart, liver, thyroid, pancreas, skin, kidney, ureter, and bladder) with the striking exception of brain. The characteristics of the interstitial dendritic cell found in heart were studied in detail, and this cell was shown to be negative for acid phosphatase, beta-glucuronidase, and ATPase activity, and certainly some and probably all of the cells were negative for nonspecific esterase activity. Experiments with colloidal carbon suggested that the cell was either poorly or not at all phagocytic. The cells were negative for surface immunoglobulin and the W3/13 antigen, but positive for the leukocyte common antigen and the SD (Class I) antigens of the major histocompatibility complex. The cell was shown to be of bone marrow origin, and either the cell itself, or more probably its precursor, was shown to be sensitive to irradiation and to cyclophosphamide. All strains tested--including the nude rat--had large numbers of interstitial dendritic cells. The widespread distribution, except in brain, of this cell, which resembles in every respect the dendritic cell described by Steinman et al. (4) in the spleen and lymph nodes of the mouse, is of interest, and the implications in this finding are discussed.
在本研究中，我们使用了一种针对大鼠Ia（RT1 - B或II类）抗原的小鼠单克隆抗体，通过对冰冻切片进行免疫荧光检测，发现在我们所检查的每个组织（心脏、肝脏、甲状腺、胰腺、皮肤、肾脏、输尿管和膀胱）的间质结缔组织中，都有强Ia阳性的树突状细胞，但脑是个显著例外。我们详细研究了心脏中发现的间质树突状细胞的特征，结果表明该细胞的酸性磷酸酶、β - 葡萄糖醛酸酶和ATP酶活性呈阴性，并且肯定有一些细胞，可能所有细胞的非特异性酯酶活性也呈阴性。用胶体碳进行的实验表明，该细胞吞噬能力很差或根本没有吞噬能力。这些细胞的表面免疫球蛋白和W3/13抗原呈阴性，但白细胞共同抗原和主要组织相容性复合体的SD（I类）抗原呈阳性。已证明该细胞起源于骨髓，并且该细胞本身或更可能是其前体细胞对辐射和环磷酰胺敏感。所有测试的品系——包括裸鼠——都有大量的间质树突状细胞。这种细胞除了在脑中之外广泛分布，在各方面都类似于Steinman等人（4）在小鼠脾脏和淋巴结中描述的树突状细胞，这一现象值得关注，并对这一发现的意义进行了讨论。
