Peng K-T, Hsu W-H, Shih H-N, Hsieh C-W, Huang T-W, Hsu R W-W, Chang P-J
Division of Sports Medicine, Department of Orthopaedic Surgery, Chang-Gung Memorial Hospital, 6 West, Chia-Pu Road, Puzih City, Chiayi 613, Taiwan.
J Bone Joint Surg Br. 2011 Sep;93(9):1201-9. doi: 10.1302/0301-620X.93B9.26736.
In this study of 41 patients, we used proteomic, Western blot and immunohistochemical analyses to show that several reactive oxygen species scavenging enzymes are expressed differentially in patients with primary osteoarthritis and those with non-loosening and aseptic loosening after total hip replacement (THR). The patients were grouped as A (n = 16, primary THR), B (n = 10, fixed THR but requiring revision for polyethylene wear) and C (n = 15, requiring revision due to aseptic loosening) to verify the involvement of the identified targets in aseptic loosening. When compared with Groups A and B, Group C patients exhibited significant up-regulation of transthyretin and superoxide dismutase 3, but down-regulation of glutathione peroxidase 2 in their hip synovial fluids. Also, higher levels of superoxide dismutase 2 and peroxiredoxin 2, but not superoxide dismutase 1, catalase and glutathione perioxidase 1, were consistently detected in the hip capsules of Group C patients. We propose that dysregulated reactive oxygen species-related enzymes may play an important role in the pathogenesis and progression of aseptic loosening after THR.
在这项针对41例患者的研究中，我们运用蛋白质组学、蛋白质印迹法和免疫组织化学分析表明，几种活性氧清除酶在原发性骨关节炎患者以及全髋关节置换术（THR）后无松动和无菌性松动患者中的表达存在差异。将患者分为A组（n = 16，初次THR）、B组（n = 10，固定THR但因聚乙烯磨损需要翻修）和C组（n = 15，因无菌性松动需要翻修），以验证所鉴定靶点在无菌性松动中的作用。与A组和B组相比，C组患者髋关节滑液中的转甲状腺素蛋白和超氧化物歧化酶3显著上调，但谷胱甘肽过氧化物酶2下调。此外，在C组患者的髋关节囊中持续检测到较高水平的超氧化物歧化酶2和过氧化物还原酶2，但超氧化物歧化酶1、过氧化氢酶和谷胱甘肽过氧化物酶1水平未升高。我们认为，活性氧相关酶的失调可能在THR后无菌性松动的发病机制和进展中起重要作用。
