School of Forest and Resource Conservation, National Taiwan University, Taipei 10617, Taiwan.
J Agric Food Chem. 2011 Oct 26;59(20):10854-9. doi: 10.1021/jf203286j. Epub 2011 Sep 30.
Three Chamaecyparis species (C. formosensis, C. obtusa, and C. obtusa var. formosana) are difficult to distinguish by the naked eye. Therefore, from the chemotaxonomic point of view, it would be valuable to find a simple and rapid method to differentiate these three Chamaecyparis species. In this study, the chemical compositions of biogenic volatile organic compounds (BVOCs) from mature leaves were analyzed using solid-phase microextraction-gas chromatography/mass spectrometry (SPME-GC/MS). Then cluster analysis (CA) and principal component analysis (PCA) were conducted for the BVOC constituents to reveal the differences among these three species. Results from SPME-GC/MS showed that the compositions of BVOCs from the three species were distinctly different. Moreover, these species were clearly differentiated according to the results of CA and PCA. In conclusion, the findings of this study suggest that SPME-GC/MS coupled with CA and PCA is a feasible and rapid technique to differentiate Chamaecyparis species with similar morphological characteristics.
三种扁柏属植物（C. formosensis、C. obtusa 和 C. obtusa var. formosana）仅凭肉眼难以区分。因此，从化学分类学的角度来看，找到一种简单快速的方法来区分这三种扁柏属植物将具有重要意义。本研究采用固相微萃取-气相色谱/质谱联用（SPME-GC/MS）法分析成熟叶片生物源挥发性有机化合物（BVOCs）的化学成分。然后对 BVOC 成分进行聚类分析（CA）和主成分分析（PCA），以揭示这三个物种之间的差异。SPME-GC/MS 的结果表明，三种物种的 BVOC 组成明显不同。此外，根据 CA 和 PCA 的结果，这些物种也得到了明确区分。总之，本研究表明，SPME-GC/MS 结合 CA 和 PCA 是一种可行且快速的技术，可以区分形态特征相似的扁柏属植物。
