[支气管哮喘患者诱导痰中HMGB1和RAGE的变化]

[Changes of HMGB1 and RAGE in induced sputum from patients with bronchial asthma].

作者信息

Cheng Zhe, Dai Ling-ling, Cao De-fei, Wu Qiu-ge, Song Yong-na, Kang Yan, Xia Jie, Si Ji-ming, Chen Chun-yan

机构信息

Department of Respiratory Medicine, Zhengzhou University, Zhengzhou, China.

出版信息

Zhonghua Yi Xue Za Zhi. 2011 Jun 14;91(22):1538-42.

PMID:21914366

Abstract

OBJECTIVE

To explore the relationship between HMGB1 (high mobility group box-1) protein and receptor for advanced glycosylation end products (RAGE) and the nosogenesis and severity of bronchial asthma.

METHODS

Based on the criteria, the asthma group included 64 acute-onset asthma patients while the control group had 20 healthy cases. The asthma group received a 4-week combination inhalation therapy of budesonide and formoterol. Lung functions and induced sputum examinations were conducted before and after treatment. The percentage of a second forced expiratory volume in the predicted value (FEV(1)%) was recorded. A differential count of neutrophilic leukocyte in reduced sputum was performed. And the sputum levels of HMGB1 and RAGE were detected by ELISA (enzyme linked immunosorbent assay).

RESULTS

Prior to treatment, the neutrophilic leukocyte percentage and the levels of HMGB1 and RAGE were all higher than those of control group (P < 0.01). The induced sputum of severe asthma patients had higher levels of neutrophilic leukocyte percentage and HMGB1 than those of mild cases (P < 0.01). But the level of RAGE showed no statistical significance among mild, moderate or severe asthma cases (P > 0.05). The post-treatment levels of neutrophilic leukocyte percentage, HMGB1 and RAGE were lower as compared with the pre-treatment ones (P < 0.01). These three parameters in uncontrolled cases were higher than those in completely controlled cases (P < 0.05); in asthma group, both HMGB1 and RAGE had a negative correlation with FEV(1)% (r = -0.830, r = -0.632, P < 0.01); in induced sputum, both HMGB1 and RAGE had a positive correlation with FEV(1)% (r = 0.820, r = 0.623, P < 0.01). The levels of HMGB1 and RAGE were positively correlated (r = 0.929, P < 0.01).

CONCLUSION

Both HMGB1 and RAGE participate in the inflammatory process of asthmatic airway. HMGB1 is correlated with the severity of asthma. And the levels of HMGB1 and RAGE in induced sputum may be employed as reference indices for the observation of therapeutic effects.

摘要

目的

探讨高迁移率族蛋白B1（HMGB1）与晚期糖基化终末产物受体（RAGE）之间的关系以及它们与支气管哮喘发病机制和严重程度的关系。

方法

根据标准，哮喘组纳入64例急性发作哮喘患者，对照组纳入20例健康者。哮喘组接受为期4周的布地奈德和福莫特罗联合吸入治疗。治疗前后进行肺功能和诱导痰检查。记录用力呼气量占预计值的百分比（FEV(1)%）。对诱导痰中嗜中性白细胞进行分类计数。采用酶联免疫吸附测定（ELISA）法检测痰中HMGB1和RAGE水平。

结果

治疗前，嗜中性白细胞百分比以及HMGB1和RAGE水平均高于对照组（P<0.01）。重度哮喘患者诱导痰中嗜中性白细胞百分比和HMGB1水平高于轻度患者（P<0.01）。但轻度、中度或重度哮喘病例中RAGE水平差异无统计学意义（P>0.05）。治疗后嗜中性白细胞百分比、HMGB1和RAGE水平较治疗前降低（P<0.01）。未控制病例的这三个参数高于完全控制病例（P<0.05）；在哮喘组中，HMGB1和RAGE与FEV(1)%均呈负相关（r=-0.830，r=-0.632，P<0.01）；在诱导痰中，HMGB1和RAGE与FEV(1)%均呈正相关（r=0.820，r=0.623，P<0.01）。HMGB1和RAGE水平呈正相关（r=0.929，P<0.01）。