拟南芥亮氨酸氨肽酶 2 的作用。
Role of the Arabidopsis leucine aminopeptidase 2.
机构信息
Department of Microbiology, Chulalongkorn University, Bangkok, Thailand.
出版信息
Plant Signal Behav. 2011 Oct;6(10):1581-3. doi: 10.4161/psb.6.10.17105. Epub 2011 Oct 1.
Abstract
Proteolysis-related genes have diverse functions across taxa and have long been considered as key players for intracellular protein turnover. Growing evidence indicates the biological significance of peptidases in degradation, maturation and modulation of bioactive peptides/proteins. By screening T-DNA tagged lines and functional analysis approaches we unraveled the Arabidopsis leucine aminopeptidase (AtLAP2) function in amino acid turnover. Transcriptomics and metabolomics profiling data suggested involvement of AtLAP2 in specific metabolic pathways. Loss-of-function of AtLAP2 resulted in early-leaf senescent and stress-sensitive phenotypes. Our work indicates an important in planta role for AtLAP2 contributing to a further understanding of the proteases having several implications in higher plants.
摘要
蛋白水解相关基因在不同分类单元中具有多样化的功能，长期以来一直被认为是细胞内蛋白质周转的关键因素。越来越多的证据表明肽酶在生物活性肽/蛋白质的降解、成熟和调节中具有重要的生物学意义。通过筛选 T-DNA 标记的品系和功能分析方法，我们揭示了拟南芥亮氨酸氨肽酶（AtLAP2）在氨基酸周转中的作用。转录组学和代谢组学分析数据表明 AtLAP2 参与了特定的代谢途径。AtLAP2 的功能丧失导致叶片早衰和对胁迫敏感的表型。我们的工作表明 AtLAP2 在植物体内具有重要作用，有助于进一步了解蛋白酶在高等植物中具有多种意义。
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