National Academy of Agricultural Science, Rural Development Administration, 225 Seodun-dong, Suwon 441-707, South Korea.
Plant Cell Rep. 2011 Jun;30(6):973-80. doi: 10.1007/s00299-010-0999-1. Epub 2011 Jan 18.
Carotenoid cleavage dioxygenases (CCDs) are involved in the production of diverse apocarotenoids including phytohormones, the visual molecules and the aromatic volatile compounds derived from carotenoids. Here, we examined the spatial expression of four of the CCD genes (AtCcd1, 4, 7 and 8) among the nine members of this family in Arabidopsis by RT-PCR. We found that the AtCcd7 gene showed strong expression in seeds. However, the promoter activity of the 1,867-bp 5'-upstream region of this gene exhibited a vascular specificity at all developmental stages throughout the transgenic Arabidopsis plants tested. The strength of the AtCcd7 promoter was also found to be lower than that of the 35S promoter by about 60%. The whole body expression of the β-glucuronidase (GUS) reporter gene driven by the AtCcd7 promoter in Arabidopsis plants was confirmed in different organs by RT-PCR and GUS enzymatic assays. Histochemical GUS staining further revealed that the AtCcd7 promoter has utility in limiting the expression of target genes to the vascular tissues in all plant organs such as the leaf, stem, root, flower and seed.
类胡萝卜素双加氧酶（CCDs）参与多种类异戊二烯的生成，包括植物激素、视觉分子和类胡萝卜素衍生的芳香挥发性化合物。在这里，我们通过 RT-PCR 研究了拟南芥中该家族的 9 个成员中的 4 个 CCD 基因（AtCcd1、4、7 和 8）的空间表达。我们发现 AtCcd7 基因在种子中表达强烈。然而，该基因 1867bp 5'上游区的启动子活性在整个转基因拟南芥植株的所有发育阶段均表现出血管特异性。AtCcd7 启动子的强度也比 35S 启动子低约 60%。通过 RT-PCR 和 GUS 酶活性测定，在不同器官中证实了由 AtCcd7 启动子驱动的β-葡萄糖醛酸酶（GUS）报告基因在拟南芥植株中的整体表达。组织化学 GUS 染色进一步表明，AtCcd7 启动子可将靶基因的表达限制在所有植物器官（如叶、茎、根、花和种子）的维管束组织中。
