Diamond R D, Noble L
Evans Memorial Department of Clinical Research, University Hospital, Boston, MA 02118.
J Infect Dis. 1990 Jul;162(1):262-5. doi: 10.1093/infdis/162.1.262.
Through guanosine triphosphate (GTP) regulatory proteins are crucial components in signal transduction by most soluble and opsonized particulate stimuli, previous data suggest that neutrophil (PMNL) activation by unopsonized hyphae differs. Most of the PMNL superoxide response evoked by unopsonized hyphae was independent of both Ca++ ions and pertussis toxin-sensitive guanine nucleotide regulatory proteins. To determine whether related regulatory proteins were involved in PMNL activation by unopsonized hyphae, separated PMNL plasma membranes were incubated with GTP and a poorly hydrolyzed, radiolabeled GTP analogue, 5'-guanylylimido-diphosphate, then stimulated. Particulate Candida albicans hyphae and soluble chemotactic peptide induced comparable guanine nucleotide release. In contrast, while unopsonized hyphae caused release, it was considerably delayed, though opsonization discernibly affected neither PMNL attachment nor spreading over hyphal surfaces. This paralleled earlier observations of other delayed responses by intact PMNL to unopsonized hyphae: phospholipase C activation, the rise in cytosolic free Ca++ ions, and actin polymerization.
通过鸟苷三磷酸（GTP）调节蛋白是大多数可溶性和调理素化颗粒刺激信号转导中的关键成分，但先前的数据表明，未调理的菌丝激活中性粒细胞（PMNL）的方式有所不同。未调理的菌丝引起的大多数PMNL超氧化物反应既不依赖于钙离子，也不依赖于百日咳毒素敏感的鸟嘌呤核苷酸调节蛋白。为了确定相关调节蛋白是否参与未调理的菌丝对PMNL的激活，将分离的PMNL质膜与GTP和一种水解缓慢的放射性标记GTP类似物5'-鸟苷酰亚胺二磷酸一起孵育，然后进行刺激。白色念珠菌颗粒菌丝和可溶性趋化肽诱导出相当的鸟嘌呤核苷酸释放。相比之下，虽然未调理的菌丝会导致释放，但释放明显延迟，不过调理作用对PMNL在菌丝表面的附着和铺展均无明显影响。这与完整的PMNL对未调理的菌丝的其他延迟反应的早期观察结果相似：磷脂酶C激活、胞质游离钙离子升高和肌动蛋白聚合。
