Lyman C A, Simons E R, Melnick D A, Diamond R D
Evans Memorial Department of Clinical Research, Boston University School of Medicine, Massachusetts.
J Infect Dis. 1988 Nov;158(5):1056-64. doi: 10.1093/infdis/158.5.1056.
Nonopsonized Candida hyphae elicit from human neutrophils a transient rise in cytosolic calcium concentrations and an oxidative burst without a detectable change in membrane potential. To determine if the signal-transduction pathway used by these organisms is mediated by guanine nucleotide-binding proteins (GNPs), we examined the functional responsiveness of neutrophils pretreated with pertussis toxin (PT). In response to serum-opsonized hyphae or zymosan, the rise in cytosolic calcium, membrane depolarization, and the respiratory burst were only partially abrogated. The transient rise in calcium induced by unopsonized hyphae was, however, completely eliminated in PT-treated neutrophils. Despite total abrogation of the calcium response, PT-treated cells could still mount a respiratory burst in response to these nonopsonized hyphae. Thus, neutrophil signaling by both serum-opsonized particles and nonopsonized hyphae is only partially mediated by PT-sensitive GNPs. Furthermore, the ability of unopsonized hyphae to elicit a respiratory burst without a calcium response suggests these events are separable and confirms the versatility of these organisms as probes for investigating neutrophil activation.
未调理的白色念珠菌菌丝可使人类中性粒细胞的胞质钙浓度短暂升高并引发氧化爆发，而膜电位无明显变化。为了确定这些生物体所使用的信号转导途径是否由鸟嘌呤核苷酸结合蛋白（GNP）介导，我们检测了用百日咳毒素（PT）预处理的中性粒细胞的功能反应性。对于血清调理的菌丝或酵母聚糖，胞质钙升高、膜去极化和呼吸爆发仅部分被消除。然而，未调理的菌丝诱导的钙短暂升高在PT处理的中性粒细胞中完全消除。尽管钙反应完全消除，但PT处理的细胞仍可对这些未调理的菌丝产生呼吸爆发。因此，血清调理颗粒和未调理菌丝引起的中性粒细胞信号传导仅部分由PT敏感的GNP介导。此外，未调理的菌丝在无钙反应的情况下引发呼吸爆发的能力表明这些事件是可分离的，并证实了这些生物体作为研究中性粒细胞活化探针的多功能性。
