University of Tartu, Institute of Chemistry, 14a Ravila Street, 50411 Tartu, Estonia.
Analyst. 2011 Nov 7;136(21):4587-94. doi: 10.1039/c1an15123a. Epub 2011 Sep 16.
Two fluoroalcohols--1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) and 1,1,1,3,3,3-hexafluoro-2-methyl-2-propanol (HFTB)--were evaluated for the first time as volatile buffer acids in the basic mobile phase for reversed-phase chromatography with electrospray ionization-mass spectrometric (LC-ESI-MS) detection of five antibiotics. Chromatographic separation as well as positive and negative ion ESI-MS intensities using these novel buffer components were compared to traditional buffer systems. Overall, the highest signal intensities and best chromatographic separation for the five antibiotics (ciprofloxacin, norfloxacin, ofloxacin, sulfadimethoxine and sulfamethoxazole) were achieved using 5 mM HFIP as the buffer acid to methanol : water mobile phase (pH of the aqueous component adjusted to 9.0 with ammonium hydroxide). Comparable results were achieved using 5 mM HFTB (pH adjusted to 9.0 with ammonium hydroxide). The suitability of HFIP for analysis of antibiotic residues in lettuce is demonstrated.
两种氟醇——1,1,1,3,3,3-六氟-2-丙醇（HFIP）和 1,1,1,3,3,3-六氟-2-甲基-2-丙醇（HFTB）——首次被评估为反相色谱中挥发性缓冲酸，用于电喷雾电离质谱（LC-ESI-MS）检测五种抗生素。使用这些新型缓冲成分的色谱分离以及正离子和负离子 ESI-MS 强度与传统缓冲系统进行了比较。总体而言，使用 5 mM HFIP 作为缓冲酸与甲醇：水流动相（通过氨水将水相的 pH 值调至 9.0），对于五种抗生素（环丙沙星、诺氟沙星、氧氟沙星、磺胺二甲氧嘧啶和磺胺甲恶唑）获得了最高的信号强度和最佳的色谱分离。使用 5 mM HFTB（通过氨水将 pH 值调至 9.0）也可获得类似的结果。HFIP 适用于分析生菜中的抗生素残留。
