Kipper K, Lillenberg M, Herodes K, Nei L, Haiba E
Institute of Chemistry, University of Tartu, Tartu, Estonia.
Estonian University of Life Sciences, Tartu, Estonia.
ScientificWorldJournal. 2017;2017:9254072. doi: 10.1155/2017/9254072. Epub 2017 Jun 12.
A simultaneous method for quantitative determination of traces of fluoroquinolones (FQs) and sulfonamides (SAs) in edible plants fertilized with sewage sludge was developed. The compounds were extracted from the plants by rapid and simple liquid extraction followed by extracts clean-up using solid phase extraction. The eluent additive 1,1,1,3,3,3-hexafluoro-2-propanol was used for liquid chromatographic detection to achieve separation of structurally similar antimicrobials like ciprofloxacin and norfloxacin. Identification and quantification of the compounds were performed using high-performance liquid chromatography with electrospray ionization mass spectrometry in selected reaction monitoring mode. Method was validated and extraction recoveries of FQs and SAs ranged from 66% to 93%. The limit of quantifications was from 5 ng/g in the case of ofloxacin to 40 ng/g for norfloxacin. The method precision ranged from 1.43% to 2.61%. The developed novel method was used to evaluate the plats antimicrobial uptake (potato L, carrot L, lettuce L, and wheat L) from soil and migration of the analytes inside the plants.
建立了一种同时测定施用污泥肥料的可食用植物中痕量氟喹诺酮类（FQs）和磺胺类（SAs）的方法。通过快速简单的液液萃取从植物中提取这些化合物，然后使用固相萃取对提取物进行净化。洗脱液添加剂1,1,1,3,3,3-六氟-2-丙醇用于液相色谱检测，以实现结构相似的抗菌药物如环丙沙星和诺氟沙星的分离。使用高效液相色谱-电喷雾电离质谱在选择反应监测模式下对化合物进行鉴定和定量。该方法经过验证，FQs和SAs的萃取回收率在66%至93%之间。定量限在氧氟沙星为5 ng/g，诺氟沙星为40 ng/g。方法精密度在1.43%至2.61%之间。所开发的新方法用于评估植物从土壤中摄取抗菌药物（马铃薯、胡萝卜、生菜和小麦）以及分析物在植物体内的迁移情况。
