Pharmaco-Toxicological Analysis Laboratory, Department of Pharmaceutical Sciences, Alma Mater Studiorum-University of Bologna, Bologna, Italy.
Electrophoresis. 2011 Oct;32(20):2809-15. doi: 10.1002/elps.201100112. Epub 2011 Sep 16.
A fast analytical method has been developed for the determination of nine amino acids, together with serotonin, in wine samples of different origin and vintage. The method is based on capillary electrophoresis coupled to laser-induced fluorescence detection. Separation was obtained by using a fused-silica capillary (75 μm id, 74.0 cm total length, 60.0 cm length to detector) and a background electrolyte composed of carbonate buffer (20 mM, pH 9.2), applying a 20 kV voltage. Direct hydrodynamic injection of wine samples was made after an original microwave-assisted derivatisation step with 5-(4,6-dichlorotriazinyl)aminofluorescein. Fluorescence was induced by an Ar-Ion laser, exciting at 488 nm. Good linearity (r(2) >0.9990) was obtained for all considered analytes and sensitivity was also good, with limits of detection in the 7-50 ng/mL range. The method was successfully applied for the analysis of commercial Italian wines and thus seems to be suitable for the determination of the relevant amino acids and serotonin, providing good results in terms of accuracy and precision, together with the advantage of a very fast, microwave-assisted derivatisation procedure. Future applications of the method are planned to check for wine adulterations and commercial frauds.
一种快速分析方法已被开发用于测定不同来源和年份的葡萄酒样品中的九种氨基酸和血清素。该方法基于毛细管电泳与激光诱导荧光检测相结合。分离是通过使用熔融硅毛细管（75μm id，74.0cm 总长度，60.0cm 至检测器长度）和由碳酸盐缓冲液（20mM，pH 9.2）组成的背景电解质来实现的，施加 20kV 电压。在原始的微波辅助衍生化步骤后，直接通过 5-(4,6-二氯三嗪基)氨基荧光素对葡萄酒样品进行水力注射。通过氩离子激光激发，在 488nm 处诱导荧光。所有考虑的分析物都具有良好的线性（r(2)>0.9990），并且灵敏度也很好，检测限在 7-50ng/mL 范围内。该方法已成功应用于商业意大利葡萄酒的分析，因此似乎适合于测定相关的氨基酸和血清素，在准确性和精密度方面提供了良好的结果，同时具有快速、微波辅助衍生化程序的优点。该方法的未来应用计划用于检查葡萄酒掺假和商业欺诈。
