Quan Xiang-Chun, Tang Hua, Ma Jing-Yun
State Key Laboratory of Water Environment Simulation of Ministry of Education, School of Environment, Beijing Normal University, Beijing 100875, China.
Huan Jing Ke Xue. 2011 Jul;32(7):2152-7.
The paper first investigated horizontal transfer of a conjugative plasmid pJP4 to two pure strains of E. coli DH5alpha and Alcaligenes sp., and a mixed culture of aerobic granular sludge, respectively. With a pJP4 receiving transconjugant Alcaligenes sp. :: pJP4 as the bioaugmented bacteria, bioaugmentation experiments were conducted in an aerobic granular sludge reactor and a biofilm reactor, respectively, to enhance the removal of a recalcitrant compound 2,4-dichlorophenoxyacetic acid(2,4-D). Results showed that pJP4 successfully transferred to E. coli DH5alpha, Alcaligenes sp. and the mixed culture of aerobic granules. For the aerobic granular sludge reactor operated in semi-continuous mode and fed with 2,4-D sole carbon source wastewater, bioaugmentation with Alcaligenes sp. : : pJP4 increased 2,4-D average removal rate significantly with an enhancement of 12% -1 498%. For the biofilm reactor operated in sequence batch mode and fed with mixed carbon sources wastewater, supplementation of the transconjugant reduced system start-up time greatly from 16 d to 5 d. It is a feasible strategy to obtain special degradative transconjugants through gene augmentation and put them into bioreactor as bioaugmentation agent to enhance the removal of some specific pollutants.
该论文首先研究了接合质粒pJP4分别向大肠杆菌DH5α和产碱杆菌的两个纯菌株以及好氧颗粒污泥混合培养物的水平转移。以获得pJP4的产碱杆菌转接合子Alcaligenes sp.::pJP4作为生物强化细菌，分别在好氧颗粒污泥反应器和生物膜反应器中进行生物强化实验，以提高难降解化合物2,4-二氯苯氧基乙酸（2,4-D）的去除率。结果表明，pJP4成功转移到大肠杆菌DH5α、产碱杆菌和好氧颗粒混合培养物中。对于以2,4-D为唯一碳源废水的半连续运行好氧颗粒污泥反应器，用Alcaligenes sp.::pJP4进行生物强化显著提高了2,4-D的平均去除率，提高幅度为12% - 1498%。对于序批式运行且以混合碳源废水为进水的生物膜反应器，添加转接合子极大地缩短了系统启动时间，从16天缩短至5天。通过基因强化获得特殊的降解性转接合子并将其作为生物强化剂投入生物反应器以提高某些特定污染物的去除率是一种可行的策略。
