Key Laboratory of Water and Sediment Sciences of Ministry of Education/State Key Laboratory of Water Environment Simulation, School of Environment, Beijing Normal University, Beijing 100875, PR China.
J Hazard Mater. 2011 Nov 30;196:278-86. doi: 10.1016/j.jhazmat.2011.09.023. Epub 2011 Sep 12.
Development of 2,4-dichlorophenoxyacetic acid (2,4-D) degrading aerobic granular sludge was conducted in two sequencing batch reactors (SBR) with one bioaugmented with a plasmid pJP4 donor strain Pseudomonas putida SM1443 and the other as a control. Half-matured aerobic granules pre-grown on glucose were used as the starting seeds and a two-stage operation strategy was applied. Granules capable of utilizing 2,4-D (about 500 mg/L) as the sole carbon source was successfully cultivated in both reactors. Gene-augmentation resulted in the enhancement of 2,4-D degradation rates by the percentage of 65-135% for the granules on Day 18, and 6-24% for the granules on Day 105. Transconjugants receiving plasmid pJP4 were established in the granule microbial community after bioaugmentation and persisted till the end of operation. Compared with the control granules, the granules in the bioaugmented reactor demonstrated a better settling ability, larger size, more abundant microbial diversity and stronger tolerance to 2,4-D. The finally obtained granules in the bioaugmented and control reactor had a granule size of around 600 μm and 500 μm, a Shannon-Weaver diversity index (H) of 0.96 and 0.55, respectively. A shift in microbial community was found during the granulation process.
采用两序批式反应器（SBR）培养 2,4-二氯苯氧乙酸（2,4-D）降解好氧颗粒污泥，其中一个生物强化接种了带有质粒 pJP4 的供体菌恶臭假单胞菌 SM1443，另一个作为对照。以半成熟的好氧颗粒污泥为接种污泥，以葡萄糖为基质，采用两阶段操作策略。在两个反应器中均成功培养出能够利用 2,4-D（约 500mg/L）作为唯一碳源的颗粒污泥。在第 18 天，基因增强使颗粒污泥的 2,4-D 降解率提高了 65-135%，在第 105 天，颗粒污泥的 2,4-D 降解率提高了 6-24%。生物强化后，在颗粒微生物群落中建立了携带质粒 pJP4 的转导体，并在运行结束时仍然存在。与对照颗粒相比，生物强化反应器中的颗粒具有更好的沉降性能、更大的粒径、更丰富的微生物多样性和更强的 2,4-D 耐受能力。生物强化和对照反应器中最终获得的颗粒粒径约为 600μm 和 500μm，香农-威纳多样性指数（H）分别为 0.96 和 0.55。在颗粒化过程中发现微生物群落发生了变化。
