Institute for Biological Sciences, Aalesund University College, Aalesund, Norway.
Vector Borne Zoonotic Dis. 2011 Oct;11(10):1329-34. doi: 10.1089/vbz.2011.0657. Epub 2011 Sep 16.
Ticks harbor a complex microbial population, which they acquire while feeding on a variety of mammalians and birds. Zoonotic diseases transferred by ticks are an increasing problem and have become a burden to the community. 16S rDNA amplification and denaturing gradient gel electrophoresis (DGGE) enables detection of the broad spectrum of bacteria that settles in the ticks. Profiling the complete microbial population in ticks may provide a better understanding of the ticks' potential to harbor and disperse pathogens. Separation of pathogenic species by DGGE is based on variation in %GC content within the 16S rDNA genetic region. Sequencing of these fragments allows identification of bacterial species. Present study identified some well-known tick-infecting bacteria, such as members of genus Borrelia, Rickettsiales, and Pseudomonas, but also less described tick-infecting bacteria such as Rhodococcus erythropolis, Spiroplasma spp., and an endosymbiont of the microarthropod Folsomia candida. This is the first report of Segniliparus rugosus-infected Ixodes ricinus ticks. Also, it is the first report of several of these pathogens in the Norwegian tick population.
蜱虫携带了复杂的微生物群体，这些微生物是它们在以各种哺乳动物和鸟类为食的过程中获得的。由蜱虫传播的人畜共患疾病是一个日益严重的问题，给社区带来了负担。16S rDNA 扩增和变性梯度凝胶电泳（DGGE）可用于检测定居在蜱虫体内的广谱细菌。分析蜱虫体内的完整微生物群体可以更好地了解蜱虫携带和传播病原体的潜力。DGGE 通过 16S rDNA 遗传区域内的%GC 含量的变化来分离致病性物种。对这些片段进行测序可鉴定细菌种类。本研究鉴定了一些众所周知的感染蜱虫的细菌，如伯氏疏螺旋体属、立克次体目和假单胞菌属的成员，但也鉴定了一些描述较少的感染蜱虫的细菌，如红球菌属、螺菌属和微小节肢动物 Folsomia candida 的内共生菌。这是首次报道感染塞氏革螨的硬蜱。此外，这也是首次在挪威蜱虫种群中报告其中几种病原体。
