Graduate School of Engineering, Iwate University, 4-3-5 Ueda, Morioka-city, Iwate 020-8551, Japan.
Int J Cosmet Sci. 2012 Feb;34(1):81-5. doi: 10.1111/j.1468-2494.2011.00684.x. Epub 2011 Sep 17.
Protein carbonyl groups in the stratum corneum may be used as a biomarker for skin photo-stress. To evaluate the relationship between the protein carbonyl to total protein (TP) ratio (carbonylation ratio) and skin photo-stress, the authors established a methodology by which protein carbonyl can be easily and highly sensitively analysed using an optical technique based on surface plasmon resonance (SPR). To collect the stratum corneum, tape stripping was employed. Firstly, the protein carbonyl was reacted with 2,4-dinitrobenzenesulfonic acid dihydrate, and the quantity of dinitrophenylated (DNP)-protein carbonyl was determined using an anti-dinitrophenyl (anti-DNP) antibody. The mass of DNP-protein carbonyl was measured using SPR. A truncated sampling-reporting cycle of <5 min allowed speedy reporting of DNP-protein carbonyl levels. A significant difference was observed in the protein carbonyl/TP ratio (carbonylation ratio) between a sun-protected area (mid-ventral arm) and a sun-exposed area (upper cheek, P < 0.05). Additionally, the carbonylation ratio of the sun-exposed area showed a higher value than that of the sun-protected area. It was suggested that the carbonylation ratio might be a useful index of skin photo-stress.
角质层中的蛋白质羰基可作为皮肤光应激的生物标志物。为了评估蛋白质羰基与总蛋白（TP）比值（羰基化比值）与皮肤光应激之间的关系，作者建立了一种使用基于表面等离子体共振（SPR）的光学技术轻松且高度灵敏地分析蛋白质羰基的方法。为了收集角质层，采用了胶带剥离法。首先，蛋白质羰基与 2,4-二硝基苯磺酸二水合物反应，并用抗二硝基苯（anti-DNP）抗体测定二硝基苯化（DNP）-蛋白质羰基的量。使用 SPR 测量 DNP-蛋白质羰基的质量。<5 分钟的截断采样报告周期允许快速报告 DNP-蛋白质羰基水平。在防晒区（中腹臂）和暴露区（上脸颊）之间，蛋白质羰基/TP 比值（羰基化比值）存在显著差异（P < 0.05）。此外，暴露区的羰基化比值显示出比防晒区更高的值。这表明羰基化比值可能是皮肤光应激的有用指标。
