Lawrence Christian
Aquatic Resources Program, Children’s Hospital Boston, Karp Family Research Laboratories, One BlackfanCircle, Boston, Massachusetts, USA.
Methods Cell Biol. 2011;104:429-51. doi: 10.1016/B978-0-12-374814-0.00023-9.
The zebrafish has emerged over the past several decades to become a mainstream animal model. While the growth of the use of the fish in basic biomedical research has been characterized by innovation, the methods and tools for fish husbandry, management, and care have been slow to evolve beyond those conceived during the initial establishment of the model system. While these approaches and technologies have certainly served the purposes of the field, they must now be improved to better match the widening scope and scale of research being done in fish. Such advances are made possible by applying new scientific information to the development of more sophisticated approaches for fish husbandry and management, and by considering the lessons learned during the establishment of the rodent model system. This review highlights the recent progress made in various areas of fish husbandry and management and points out new directions for further improvements.
在过去几十年里，斑马鱼已成为一种主流动物模型。虽然在基础生物医学研究中使用这种鱼的增长以创新为特征，但鱼类养殖、管理和护理的方法及工具，除了在模型系统最初建立时构想的那些之外，发展缓慢。虽然这些方法和技术肯定满足了该领域的需求，但现在必须加以改进，以更好地适应在鱼类中进行的研究不断扩大的范围和规模。通过将新的科学信息应用于开发更复杂的鱼类养殖和管理方法，并借鉴在啮齿动物模型系统建立过程中吸取的经验教训，这些进展才得以实现。本综述重点介绍了鱼类养殖和管理各个领域的最新进展，并指出了进一步改进的新方向。
