An alternative quenched fluorescence substrate for Pz-peptidase.
作者信息
Tisljar U, Knight C G, Barrett A J
机构信息
Biochemistry Department, Strangeways Research Laboratory, Cambridge, United Kingdom.
出版信息
Anal Biochem. 1990 Apr;186(1):112-5. doi: 10.1016/0003-2697(90)90582-t.
PMID:2192576
Abstract
7-Methoxycoumarin-3-carboxylyl-Pro-Leu-Gly-Pro-D-Lys(2,4-dinitr oph enyl) is introduced as a new quenched fluorescence substrate for assaying Pz-peptidase (also known as soluble metallo-endopeptidase and endo-oligopeptidase). The value of Km for partially purified Pz-peptidase from rat muscle was 8.6 microM. High protein concentrations did not interfere with the assay, so that for the first time continuous assays of Pz-peptidase in crude tissue extracts became possible.
摘要
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