Barrett A J, Knight C G, Brown M A, Tisljar U
Biochemistry Department, Strangeways Research Laboratory, Cambridge, U.K.
Biochem J. 1989 May 15;260(1):259-63. doi: 10.1042/bj2600259.
The peptide derivative N alpha-(2,4-dinitrophenyl)-L-prolyl-L-leucyl-glycyl-L-prolyl-L-tryptophanyl-D- lysine (Dnp-Pro-Leu-Gly-Pro-Trp-D-Lys) has been found to be a convenient substrate for the assay of clostridial collagenase and Pz-peptidase. The substrate shows a 25-fold enhancement of fluorescence (gamma ex. 283 nm, lambda em. 350 nm) following hydrolysis of the Leu2-Gly3 peptide bond. The value of Km for clostridial collagenase was 17 microM. The substrate for the first time makes possible continuous fluorimetric assays for Pz-peptidase and clostridial collagenase.
已发现肽衍生物Nα-(2,4-二硝基苯基)-L-脯氨酰-L-亮氨酰-甘氨酰-L-脯氨酰-L-色氨酰-D-赖氨酸(Dnp-Pro-Leu-Gly-Pro-Trp-D-Lys)是用于检测梭菌胶原酶和Pz肽酶的便捷底物。在Leu2-Gly3肽键水解后,该底物的荧光增强了25倍(激发波长283nm,发射波长350nm)。梭菌胶原酶的米氏常数(Km)为17μM。该底物首次使对Pz肽酶和梭菌胶原酶进行连续荧光测定成为可能。