Sakamoto H, Sasaki S, Hirata Y, Imai T, Ando K, Ida T, Sakurai T, Yanagisawa M, Masaki T, Marumo F
Second Department of Internal Medicine, Tokyo Medical and Dental University, Yushima, Tokyo, Japan.
Biochem Biophys Res Commun. 1990 Jun 15;169(2):462-8. doi: 10.1016/0006-291x(90)90354-p.
We investigated whether ET-1 is synthesized by and released from mesangial cells. ET-1-like immunoreactivity (LI) released into medium increased time-dependently under a serum-free condition. The amounts of ET-1-LI released into the medium was augmented in the presence of fetal calf serum. Reverse-phase HPLC profile of the conditioned media revealed a major component coeluting with standard ET-1. Northern blot analysis of poly(A) +RNA extracted from mesangial cells showed a single major band corresponding to the size of preproET-1 mRNA (2.3 kb). These findings demonstrate that ET-1 is synthesized by and released from rat mesangial cells and suggest a possibility that it acts on their own cells as an autocrine factor.
我们研究了内皮素 -1（ET-1）是否由系膜细胞合成并释放。在无血清条件下，释放到培养基中的ET-1样免疫反应性（LI）随时间呈依赖性增加。在胎牛血清存在的情况下，释放到培养基中的ET-1-LI量增加。条件培养基的反相高效液相色谱图谱显示，有一个主要成分与标准ET-1共洗脱。对从系膜细胞中提取的聚腺苷酸（poly(A)）+RNA进行的Northern印迹分析显示，有一条单一的主要条带，其大小与前内皮素原-1 mRNA（2.3 kb）相对应。这些发现表明，ET-1由大鼠系膜细胞合成并释放，并提示其有可能作为自分泌因子作用于自身细胞。
