Nunez D J, Taylor E A, Oh V M, Schofield J P, Brown M J
Clinical Pharmacology Unit, Addenbrooke's Hospital, Cambridge, U.K.
Biochem J. 1991 May 1;275 ( Pt 3)(Pt 3):817-9. doi: 10.1042/bj2750817.
Cultured pig and bovine endothelial cells are capable of synthesizing endothelin-1 (ET-1). Thus the observation that the kidney contains a large number of binding sites for ET distributed in close proximity to endothelial cells suggests that ET-1 may be released from the endothelium to act locally on these receptors. In support of this hypothesis, using the technique of reverse transcription with specific amplification of cDNA, we report here that ET-1 mRNA is expressed in the rat kidney. The partial sequence of the amplified rat ET-1 cDNA confirms that the mature rat peptide is identical to that of the mouse, man and pig, but with some differences in codon usage.
培养的猪和牛内皮细胞能够合成内皮素-1（ET-1）。因此，肾脏含有大量与内皮细胞紧邻分布的ET结合位点这一观察结果表明，ET-1可能从内皮释放出来，在局部作用于这些受体。为支持这一假说，我们运用逆转录结合cDNA特异性扩增技术，在此报告ET-1 mRNA在大鼠肾脏中表达。扩增的大鼠ET-1 cDNA的部分序列证实，成熟的大鼠肽与小鼠、人类和猪的相同，但密码子使用存在一些差异。
