Diabetes and Obesity Program, Garvan Institute of Medical Research , Sydney, NSW 2010, Australia.
J Proteome Res. 2011 Nov 4;10(11):4970-82. doi: 10.1021/pr200446r. Epub 2011 Oct 18.
The adipocyte is a key regulator of mammalian metabolism. To advance our understanding of this important cell, we have used quantitative proteomics to define the protein composition of the adipocyte plasma membrane (PM) in the presence and absence of insulin. Using this approach, we have identified a high confidence list of 486 PM proteins, 52 of which potentially represent novel cell surface proteins, including a member of the adiponectin receptor family and an unusually high number of hydrolases with no known function. Several novel insulin-responsive proteins including the sodium/hydrogen exchanger, NHE6 and the collagens III and VI were also identified, and we provide evidence of PM-ER association suggestive of a unique functional association between these two organelles in the adipocyte. Together these studies provide a wealth of potential therapeutic targets for the manipulation of adipocyte function and a valuable resource for metabolic research and PM biology.
脂肪细胞是哺乳动物代谢的关键调节者。为了深入了解这种重要的细胞，我们利用定量蛋白质组学技术，在存在和不存在胰岛素的情况下，定义了脂肪细胞膜（PM）的蛋白质组成。通过这种方法，我们确定了一个高可信度的 486 种 PM 蛋白列表，其中 52 种可能代表新的细胞表面蛋白，包括脂联素受体家族的一个成员和数量异常多的具有未知功能的水解酶。还鉴定出了几个新的胰岛素反应蛋白，包括钠/氢交换器 NHE6 和胶原 III 和 VI，并且我们提供了 PM-ER 关联的证据，提示这两个细胞器在脂肪细胞中有独特的功能关联。这些研究共同为脂肪细胞功能的操纵提供了丰富的潜在治疗靶点，并为代谢研究和 PM 生物学提供了宝贵的资源。
