Schmezer P, Pool B L, Lefevre P A, Callander R D, Ratpan F, Tinwell H, Ashby J
Institute of Toxicology and Chemotherapy, German Cancer Research Centre, Heidelberg, Federal Republic of Germany.
Environ Mol Mutagen. 1990;15(4):190-7. doi: 10.1002/em.2850150404.
N-Nitrosodibenzylamine (NDBzA) is mutagenic to Salmonella typhimurium and induces DNA strand breaks in isolated rat hepatocytes, yet it is reported to be non-carcinogenic to the rat. Here we report that it is inactive in both the rat and mouse bone marrow micronucleus assays and in a rat liver autoradiographic assay for unscheduled DNA synthesis. It is, however, clearly active as a micronucleus-inducing agent and mitogen in the rat liver and is capable of inducing single-strand breaks in the DNA of rat liver. The origin and implications of this curious conflict of in vivo genotoxicity data are discussed. Irrespective of that discussion, it is concluded that NDBzA is genotoxic to the rat liver in vivo.
N-亚硝基二苄胺（NDBzA）对鼠伤寒沙门氏菌具有致突变性，并能在分离的大鼠肝细胞中诱导DNA链断裂，但据报道它对大鼠无致癌性。在此我们报告，它在大鼠和小鼠骨髓微核试验以及大鼠肝脏非程序性DNA合成的放射自显影试验中均无活性。然而，它在大鼠肝脏中作为微核诱导剂和有丝分裂原具有明显活性，并且能够在大鼠肝脏DNA中诱导单链断裂。本文讨论了这种体内遗传毒性数据奇怪冲突的起源及影响。无论该讨论如何，得出的结论是NDBzA在体内对大鼠肝脏具有遗传毒性。
