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在振荡流中通过溶剂弹性实现DNA的拉伸与迁移

Stretching and migration of DNA by solvent elasticity in an oscillatory flow.

作者信息

Lo Wei-Chang, Hong H C, Choi H J, Lai Pik-Yin, Chan C K

机构信息

Institute of Physics, Academia Sinica, Nankang, Taipei 115, Taiwan, Republic of China.

出版信息

Phys Rev E Stat Nonlin Soft Matter Phys. 2011 Aug;84(2 Pt 1):021802. doi: 10.1103/PhysRevE.84.021802. Epub 2011 Aug 4.

Abstract

A model with solution viscoelasticity is proposed to explain the ratchetlike stretching of DNA by a symmetric ac electric field in polymer solutions. In this model, DNA is stretched by the interaction between the fluid elasticity and the oscillatory flow induced by DNA. Predictions of the model are confirmed by DNA stretching experiments performed in various polymer solutions and the corresponding rheological measurements of the solutions. In particular, experiments have verified that a net migration of stretched DNA in polymer solutions can be induced by a zero-mean asymmetric ac electric field. This last finding cannot be explained by other existing models.

摘要

提出了一种具有溶液粘弹性的模型,以解释聚合物溶液中对称交流电场对DNA的棘轮状拉伸。在该模型中,DNA通过流体弹性与DNA诱导的振荡流之间的相互作用而被拉伸。在各种聚合物溶液中进行的DNA拉伸实验以及溶液相应的流变学测量证实了该模型的预测。特别是,实验已经证实,零均值非对称交流电场可以诱导聚合物溶液中拉伸的DNA发生净迁移。这一最新发现无法用其他现有模型来解释。

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