College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.
Genome. 2011 Oct;54(10):812-8. doi: 10.1139/g11-047. Epub 2011 Sep 19.
The octamer-binding transcription factor 4 gene (Oct4) plays a critical role in maintaining pluripotency during early mammalian embryonic development and self-renewal of embryonic stem (ES) cells. In this study, we cloned the Oct4 cDNA and 2.8-kb regulatory region upstream of the start codon in Guanzhong dairy goat ( Capra hircus ). The comparative sequence analysis of Oct4 cDNA showed that it was highly conserved among six mammalian species. The goat Oct4 5' regulatory regions were homologous to the corresponding regions of Oct4 in other species and were functional in directing the expression of luciferase in mouse P19 embryonic carcinoma cells and mouse J1 ES cells. Furthermore, the methylation levels in the goat Oct4 minimal promoter and proximal enhancer in the fetal genital ridge were lower than those in the heart. Additionally, two processed pseudogenes that shared high homology with goat Oct4 cDNA were identified and characterized.
八聚体结合转录因子 4 基因（Oct4）在维持早期哺乳动物胚胎发育中的多能性和胚胎干细胞（ES 细胞）的自我更新中起着关键作用。在这项研究中，我们克隆了关中奶山羊（Capra hircus）的 Oct4 cDNA 和起始密码子上游的 2.8kb 调控区。Oct4 cDNA 的比较序列分析表明，它在六种哺乳动物物种中高度保守。山羊 Oct4 的 5'调控区与其他物种的 Oct4 相应区域同源，并能在指导荧光素酶在小鼠 P19 胚胎癌细胞和小鼠 J1 ES 细胞中的表达。此外，胎生生殖脊中山羊 Oct4 最小启动子和近端增强子的甲基化水平低于心脏中的甲基化水平。此外，还鉴定并表征了两个与山羊 Oct4 cDNA 具有高度同源性的加工假基因。
