Zucchelli G C, Pilo A, Masini S, Chiesa M R, Prontera C
Istituto di Fisiologia Clinica, Consiglio Nazionale delle Ricerche, Pisa, Italy.
J Clin Chem Clin Biochem. 1990 Apr;28(4):193-7. doi: 10.1515/cclm.1990.28.4.193.
A recently developed chemiluminescence immunoassay system (LIA-mat) for triiodothyronine and thyroxine, set up by Byk-Sangtec Diagnostica (Dietzenbach, Germany), has been evaluated and compared with radioimmunoassays and with a chemiluminescence enhanced enzyme immunoassay (Amerlite), using control materials circulated in a national interlaboratory quality control, as well as patient sera. The LIA-mat assays are competitive methods which use coated monoclonal antibodies and triiodothyronine- or thyroxine-ABEI (aminobutylethylisoluminol) conjugate as tracers. The working range of LIA-mat T3 (computed from the within-assay precision profile) extended from 1.4 to 12.3 nmol/l; the between-assay precision was 8.1 - 19.3 CV%. Regression analysis of the LIA-mat T3 results (y) against the consensus means (x) of the participants in the national interlaboratory survey yielded: y = -0.14 + 1.05 x, r = 0.95. The working range of LIA-mat T4 extended from 33 to 515 nmol/l; the between-assay precision was 5.4 - 9.2 CV%. An excellent agreement was found between LIA-mat T4 results (y) and the consensus means (x) of the laboratories participating in the national interlaboratory survey (y = 3.79 + 1.02 x, r = 0.98).
由德国迪岑巴赫的拜克-桑泰克诊断公司研发的一种用于三碘甲状腺原氨酸和甲状腺素的化学发光免疫分析系统（LIA-mat），已通过使用全国实验室间质量控制中流通的对照材料以及患者血清，与放射免疫分析和化学发光增强酶免疫分析（Amerlite）进行了评估和比较。LIA-mat分析是竞争法，使用包被的单克隆抗体和三碘甲状腺原氨酸或甲状腺素-ABEI（氨基丁基乙基异鲁米诺）偶联物作为示踪剂。LIA-mat T3的工作范围（根据批内精密度曲线计算）为1.4至12.3 nmol/l；批间精密度为8.1 - 19.3 CV%。对全国实验室间调查参与者的LIA-mat T3结果（y）与共识均值（x）进行回归分析，得到：y = -0.14 + 1.05 x，r = 0.95。LIA-mat T4的工作范围为33至515 nmol/l；批间精密度为5.4 - 9.2 CV%。在参与全国实验室间调查的实验室的LIA-mat T4结果（y）与共识均值（x）之间发现了极好的一致性（y = 3.79 + 1.02 x，r = 0.98）。
