Shahriari Shahriar, Rezaei Aliasghar, Jalalzadeh Seyed Mohsen, Mani Khosro, Zamani Alireza
Department of Immunology-Hematology, Hamadan University of Medical Sciences, Hamadan, Iran.
Iran J Immunol. 2011 Sep;8(3):176-82.
Bone resorption is one of the main features of inflammatory periapical lesions and is mainly mediated by interleukin-1 beta (IL-1β), tumor necrosis factor-alpha (TNF-α) and prostaglandin-E2 (PGE2). Recent investigations of these lesions revealed that pharmacological modulation may be possible.
The aim of this study was to evaluate the effect of Ibuprofen on IL-1β, TNF-α and PGE2 levels in periapical exudates and compare the results with a group of placebo control.
Thirty patients with non vital teeth and radiographic lesions were divided into two groups of case and control according to their entrance to the study. Periapical exudates were taken from root canals using absorbent paper points and followed by 400 mg Ibuprofen and placebo prescribed one tablet every 6 hour for three days and in the fourth day second samples were taken, then final cleaning, shaping and obturation of the canals were completed. IL-1β, TNF-α and PGE2 levels were determined by enzyme-linked immunosorbent assays (ELISA). Data were analyzed using paired t-test and student's t-test.
The results showed that PGE2 levels were decreased significantly in the case group to 86.92 ± 72.42 Pg/ml following Ibuprofen treatment comparing with the pre-treatment (164.96 ± 12.255 Pg/ml) (p=0.02) and placebo group (154.2 ± 97.13 Pg/ml) (p=0.001). But there were no significant differences in IL-1β and TNF-α level between the two groups and in each group before and after treatment.
The data indicate that Ibuprofen, as a non-steroidal anti-inflammatory drug (NSAID), can be used to block PGE2 release, enhance healing of inflammatory periapical lesions and possibly to inhibit bone resorption.
骨吸收是炎性根尖周病变的主要特征之一，主要由白细胞介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）和前列腺素-E2（PGE2）介导。最近对这些病变的研究表明，进行药物调节或许是可行的。
本研究旨在评估布洛芬对根尖周渗出物中IL-1β、TNF-α和PGE2水平的影响，并将结果与一组安慰剂对照组进行比较。
30例患有无活力牙且有影像学病变的患者根据进入研究的先后顺序分为病例组和对照组。使用吸潮纸尖从根管中获取根尖周渗出物，随后给予400毫克布洛芬和安慰剂，每6小时服用1片，持续3天，在第4天采集第二次样本，然后完成根管的最终清理、预备和充填。通过酶联免疫吸附测定（ELISA）法测定IL-1β、TNF-α和PGE2水平。采用配对t检验和学生t检验对数据进行分析。
结果显示，与治疗前（164.96±12.255皮克/毫升）（p=0.02）和安慰剂组（154.2±97.13皮克/毫升）（p=0.001）相比，病例组在布洛芬治疗后PGE2水平显著降低至86.92±72.42皮克/毫升。但两组之间以及每组治疗前后的IL-1β和TNF-α水平均无显著差异。
数据表明，布洛芬作为一种非甾体抗炎药（NSAID），可用于阻断PGE2释放，促进炎性根尖周病变的愈合，并可能抑制骨吸收。
