Fang Jian-qiao, Liu Fang, Shao Xiao-mei, Wu Yuan-yuan
The Third Clinical Medical School, Zhejiang Chinese Medical University, Hangzhou 310053, China.
Zhen Ci Yan Jiu. 2007 Aug;32(4):224-8.
To observe the anti-inflammatory effects of electroacupuncture (EA) at "Quchi" (LI 11) and its influence on IL-1beta, TNF-alpha concentrations and mRNA expressions in carrageenan (CAR)-induced inflammation rats, so as to reveal partial mechanisms of EA in relieving acute inflammation.
Sixty male Wistar rats were divided into control, model, Indomethacin (IND, 7.5 mg/kg, oral perfusion), Rofecoxib (7.5 mg/kg, oral perfusion), EA-pre-treatment (EA was given once daily for 5 days before modeling), EA-post-treatment (EA was given once after modeling) groups, with 10 cases in each. EA (2 Hz, 5 mA and persistent waves) was applied to "Quchi" (LI 11) for 30 min each time. Arthritis model was established by subcutaneous injection of 1% CAR, 0.1 mL into the right hind paw. Paw edema degree of the affected hind paw was measured with a foot-volume surveyor. After processing samples of the affected paw toes (including smashing, centrifugalization, etc.), concentrations of prostaglandin (PG) E2, interleukin (IL)-1beta, tumor necrosis factor (TNF)-alpha were measured by enzyme linked immunosorbent assay (ELISA). Expressions of IL-1beta mRNA and TNF-alpha mRNA in the local inflammatory tissues were detected by reverse-transcription polymerase chain reaction (RT-PCR) assay.
Compared with model group, the affected hind-paw-volume decreased significantly from 2 h on in EA-post-treatment group and from 1 h on in IND group after injection of CAR. Compared with control group, PGE2, IL-1beta and TNF-alpha contents in the toe tissue of model group increased significantly; while in comparison with model group, PGE2 and IL-1beta levels in EA-post-treatment group, IL-1beta level in IND group decreased significantly (P < 0.05), and TNF-alpha level in IND group increased significantly. No significant differences were found between model group and Rofecoxib and EA-pre-treatment groups in most time-courses of the hind-paw-volume, between model and IND, Rofecoxib and EA-pre-treatment groups in PGE2, between model and EA-pre-treatment groups in IL-1beta, and between model and Rofecoxib, EA-pre-treatment and EA-post-treatment groups in TNF-alpha contents (P > 0.05). Compared with model group, IL-1beta mRNA expression of the local inflammatory tissues in IND and EA-post-treatment groups was down-regulated, and TNF-alpha mRNA expression in Rofecoxib and two EA groups down-regulated slightly.
EA possesses anti-inflammatory effect in acute arthritis rats, which is closely related to its effects in reducing the secretion of PGE2, IL-1beta and TNF-alpha and the expression of IL-1beta mRNA and TNF-alpha mRNA.
观察电针“曲池”（LI 11）的抗炎作用及其对角叉菜胶（CAR）致炎大鼠白细胞介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）浓度及mRNA表达的影响，以揭示电针缓解急性炎症的部分机制。
将60只雄性Wistar大鼠分为对照组、模型组、吲哚美辛（IND，7.5 mg/kg，灌胃）组、罗非昔布（7.5 mg/kg，灌胃）组、电针预处理组（造模前每日电针1次，共5天）、电针后处理组（造模后电针1次），每组10只。每次将电针（2 Hz，5 mA，连续波）作用于“曲池”（LI 11）30分钟。通过右后足皮下注射1% CAR 0.1 mL建立关节炎模型。用足容积测量仪测量患侧后足爪水肿程度。处理患侧爪趾样本（包括捣碎、离心等）后，采用酶联免疫吸附测定（ELISA）法检测前列腺素（PG）E2、白细胞介素（IL）-1β、肿瘤坏死因子（TNF）-α浓度。采用逆转录聚合酶链反应（RT-PCR）法检测局部炎症组织中IL-1β mRNA和TNF-α mRNA的表达。
与模型组比较，注射CAR后，电针后处理组患侧后足爪容积从2小时起显著降低，IND组从1小时起显著降低。与对照组比较，模型组趾组织中PGE2、IL-1β和TNF-α含量显著升高；与模型组比较，电针后处理组PGE2和IL-1β水平、IND组IL-1β水平显著降低（P < 0.05），IND组TNF-α水平显著升高。在患侧后足爪容积的大多数时间点上，模型组与罗非昔布组和电针预处理组之间、模型组与IND组、罗非昔布组和电针预处理组在PGE2方面、模型组与电针预处理组在IL-1β方面、模型组与罗非昔布组、电针预处理组和电针后处理组在TNF-α含量方面比较，差异均无统计学意义（P > 0.05）。与模型组比较，IND组和电针后处理组局部炎症组织中IL-1β mRNA表达下调，罗非昔布组和两个电针组中TNF-α mRNA表达略有下调。
电针对急性关节炎大鼠具有抗炎作用，这与其降低PGE2、IL-1β和TNF-α的分泌以及IL-1β mRNA和TNF-α mRNA的表达密切相关。
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