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一个通过偶然发现克隆自拟餐鲦复合体(硬骨鱼纲,鲤科)的多态微卫星,对于多倍体的遗传分析可能是有用的。

A polymorphic microsatellite from the Squalius alburnoides complex (Osteichthyes, Cyprinidae) cloned by serendipity can be useful in genetic analysis of polyploids.

机构信息

Departamento de Biodiversidad y Biología Evolutiva, Museo Nacional Ciencias Naturales, CSIC, Madrid, Spain.

出版信息

Genet Mol Biol. 2011 Jul;34(3):524-8. doi: 10.1590/S1415-47572011005000023. Epub 2011 Jul 1.

Abstract

A new microsatellite locus (SAS1) for Squalius alburnoides was obtained through cloning by serendipity. The possible usefulness of this new species-specific microsatellite in genetic studies of this hybrid-species complex, was explored. The polymorphism exhibited by SAS1 microsatellite is an important addition to the set of microsatellites previously used in genetic studies in S. alburnoides complex, that mostly relied in markers described for other species. Moreover, the SAS1 microsatellite could be used to identify the parental genomes of the complex, complementing other methods recently described for the same purpose..

摘要

通过偶然发现,从黄黝鱼中克隆到一个新的微卫星位点(SAS1)。探讨了这个新的种特异性微卫星在这个杂交种复合体遗传研究中的可能用途。SAS1 微卫星表现出的多态性是对之前用于黄黝鱼复合体遗传研究的微卫星集合的重要补充,这些微卫星主要依赖于为其他物种描述的标记。此外,SAS1 微卫星可用于鉴定复合体的亲本基因组,补充了最近为同一目的描述的其他方法。

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