School of Biosciences, University of Exeter, Exeter EX4 4PS, UK.
Mol Ecol Resour. 2008 Mar;8(2):462-5. doi: 10.1111/j.1471-8286.2007.01994.x.
In order to identify microsatellite loci for parentage analysis in roach Rutilus rutilus, 59 published primer sets were tested on roach and eight other cyprinid fish. Twenty polymorphic loci were identified for roach, of which the polymerase chain reaction products of seven could be pooled for sequencer analysis. Together, these seven loci have an exclusion probability of 0.997 for parentage, when no parents are known. We also describe a novel test for hybrids between roach and four other cyprinids, based on intraspecies length differences of internal transcribed spacer region 1.
为了鉴定罗非鱼（Rutilus rutilus）亲权分析的微卫星位点，我们在罗非鱼和其他 8 种鲤科鱼类上测试了 59 对已发表的引物。在罗非鱼中鉴定出 20 个多态性位点，其中 7 个聚合酶链反应产物可合并进行测序分析。当没有亲本信息时，这 7 个位点的亲权排除概率为 0.997。我们还描述了一种新的方法来检测罗非鱼和其他 4 种鲤科鱼类之间的杂种，基于内转录间隔区 1 的种内长度差异。
