College of Fisheries, Ocean University of China, Qingdao 266003, China.
Mar Biotechnol (NY). 2012 Apr;14(2):218-26. doi: 10.1007/s10126-011-9405-4. Epub 2011 Sep 20.
The Pacific oyster (Crassostrea gigas) is one of the most important oysters cultured worldwide. To analyze the oyster genome and dissect growth-related traits, we constructed a sex-averaged linkage map by combining 64 genomic simple sequence repeats, 42 expressed sequence tag-derived SSRs, and 320 amplified fragment length polymorphism markers in an F(1) full-sib family. A total of 426 markers were assigned to 11 linkage groups, spanning 558.2 cM with an average interval of 1.3 cM and 94.7% of genome coverage. Segregation distortion was significant for 18.8% of the markers (P < 0.05), and distorted markers tended to occur on some genetic regions or linkage groups. Most growth-related quantitative traits were highly significantly (P < 0.01) correlated, and principal component analysis obtained four principal components. Quantitative trait locus (QTL) analysis identified three significant QTLs for two principal components, which explained 0.6-13.9% of the phenotypic variation. One QTL for sex was detected on linkage group 6, and the inheritabilities of sex for parental alleles and maternal alleles on that locus C15 are 39.8% and 0.01%, respectively. The constructed linkage map and determined QTLs can provide a tool for further genetic analysis of the traits and be potential for marker-assisted selection in C. gigas breeding.
太平洋牡蛎（Crassostrea gigas）是全球最重要的养殖牡蛎之一。为了分析牡蛎基因组并剖析生长相关的性状，我们通过在一个 F1 全同胞家系中组合 64 个基因组简单序列重复、42 个表达序列标签衍生的 SSR 和 320 个扩增片段长度多态性标记，构建了一个平均连锁图谱。总共 426 个标记被分配到 11 个连锁群，总长 558.2 cM，平均间隔为 1.3 cM，基因组覆盖率为 94.7%。18.8%的标记存在显著的分离失真（P < 0.05），且失真标记倾向于出现在某些遗传区域或连锁群上。大多数生长相关的数量性状高度显著（P < 0.01）相关，主成分分析得到了四个主成分。数量性状位点（QTL）分析鉴定了两个主成分的三个显著 QTL，这些 QTL 解释了 0.6-13.9%的表型变异。在连锁群 6 上检测到一个性别 QTL，该位点 C15 上的亲代等位基因和母本等位基因的性别遗传率分别为 39.8%和 0.01%。构建的连锁图谱和确定的 QTL 可以为进一步分析这些性状的遗传分析提供工具，并为 C. gigas 育种中的标记辅助选择提供潜力。
